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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

Receptor Binding Assays

MultiScreen_HTS plates provide the benefits of filtration in a convenient, automation-friendly, 96-well format. More

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Overview

Specifications

Ordering Information

96-well MultiScreen_HTS Plates with Durapore Membrane (PVDF)Clear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pore Size	Pack Size
MSGVN2B50	MultiScreen_HTS GV Filter Plate, 0.22 µm, opaque, non-sterile	0.22 µm	50
MSHVN4B50	MultiScreen_HTS HV Filter Plate, 0.45 µm, opaque, non-sterile	0.45 µm	50
MSDVN6B50	MultiScreen_HTS DV Filter Plate, 0.65 µm, opaque, non-sterile	0.65 µm	50

384-well MultiScreen_HTS Plates with Durapore Membrane (PVDF)Clear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pore Size	Pack Size
MZHVN0W10	MultiScreen_HTS 384 well	0.45 µm	10
MZHVN0W50	MultiScreen_HTS384 well	0.45 µm	50

96-well MultiScreen_HTS Plates with Glass Fiber FilterClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pore Size	Pack Size
MSFBN6B10	MultiScreen_HTS FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile	1.0 / 0.65 µm	10
MSFBN6B50	MultiScreen_HTS FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile	1.0 / 0.65 µm	50
MSFBNXB50	MultiScreen_HTS+ Hi Flow FB Filter Plate	1.0 / 0.65 µm	50
MSFCN6B10	MultiScreen_HTS FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile	1.2 / 0.65 µm	10
MSFCN6B50	MultiScreen_HTS FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile	1.2 / 0.65 µm	50
MSFCNXB50	MultiScreen_HTS+ FC Filter Plate, Hi Flow, opaque, non-sterile	1.2 / 0.65 µm	50

384-well MultiScreen_HTS Plates with Glass Fiber FilterClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pore Size	Pack Size
MZFBN0W10	MultiScreen_HTS 384 well	1.0 / 0.65 µm	10
MZFBN0W50	MultiScreen_HTS 384 well	1.0 / 0.65 µm	50
MZFCN0W10	MultiScreen_HTS 384 well	1.2 / 0.65 µm	10
MZFCN0W50	MultiScreen_HTS 384 well	1.2 / 0.65 µm	50

MultiScreen Harvest PlatesClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pore Size	Pack Size
MAHFB1H60	MultiScreen 96 well Harvest	1.0 / 0.65 µm	60
MAHFC1H60	MultiScreen 96 well Harvest	1.2 / 0.65 µm	60

AccessoriesClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
MSVMHTS00	MultiScreen_HTS Vacuum Manifold	1
MAVM0960R	MultiScreen™ Vacuum Manifold 96-well	1
MATAHCL00	MultiScreen Sealing Tape, clear, non-sterile	100
MSTPCWH50	Packard Top Count Adapter for MultiScreen_HTS 96-well plate	50

Documentation

References

Reference overview	Application
Receptor binding assay for nitric oxide- and heme-independent activators of soluble guanylate cyclase. Peter Schmidt, Matthias Schramm, Henning Schroder and Johannes-Peter Stasch Analytical Biochemistry 314 (1): 162-165 2003
High-throughput receptor-binding methods for somatostatin receptor 2 Birin ET, Rohrer SP,Anal. Biochem. 2002 Aug 1; 307 (1):159-66 Anal. Biochem. 2002 Aug 1; 307 (1):159-66 2002
Quantitative and functional characterization of muscarinic receptor subtypes in insulin-secreting cell lines and rat pancreatic islets Iismaa, Tiina P. et al. Diabetes, 49, 3, 392, 2000 2000	Cell Harvesting, Receptor/Ligand Binding Assays
Development of 5-hydroxytryptamine(2A) receptor binding assay for high throughput screening using 96-well microfilter plates Harms A, Gundisch D, Muller CE, Kovar KA J Biomol Screen. 2000 Aug;5(4):269-78 2000
Islet cell membrane antigens activate diabetogenic (CD4.sup. +) T-cells in BB/Wor rat Ellerman, Karen E. et al. Diabetes, 48, 5, 975, 1999 1999	Cell Harvesting, Receptor/Ligand Binding Assays
Quantitative cell membrane-based radioligand binding assays for parathyroid hormone receptors. Sam R. J. Hoare and Ted B. Usdin Journal of Pharmacological and Toxicological Methods 41 (2-3): 83-90 1999
An immunoassay for assessment of receptor tyrosine kinase autophosphorylation Grace R. Nakayama, Zahra Parandoosh Journal of immunological methods 225 (1999), 67-74 1999
Synthesis and Structure-Activity Relationship of a New Series of Potent AT1 Selective Angiotensin II Receptor Antagonists: 5-(Biphenyl-4-ylmethyl) pyrazoles Carmen, A., Gomez, L.A., Cavalcanti, F.L., deArriba, A.F., Garcia-Rafanell, J., and Forn, J. J. Med. Chemical 40: 547-558 1997	Cell Harvesting, Receptor/Ligand Binding Assays
Mutagenesis of Acidic Residues in the Oxygenase Diomain of Inducible Nitric-Oxide Synthase Identifies a Glutamate Involved in Arginine Binding Gachhui, R., Ghosh, D.K., Wu, C., Parkinson, J., Crane, B.R., Stuehr, D.J. BioChemistry. 36:17. 5097 - 5103 1997	Cell Harvesting, Receptor/Ligand Binding Assays
Molecular Cloning of Murine CC CKR-4 and High Affinity Binding of Chemokines to Murine and Human CC CKR-4 Hoogewerf, A.J., Black, D., Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. Biochemical and Biophysical Research Communications, 218, 337-343 1996	Cell Harvesting, Receptor/Ligand Binding Assays

Related Products & Applications

Categories

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates

C7747MultiScreen_HTS plates provide the benefits of filtration in a convenient, automation-friendly, 96-well format.

Receptor-binding assays are a critical component in lead indentification and later lead characterization processes. They are used to characterize most known drug targets and typically use
filter-based separations technology to obtain necessary “bound vs. free” fractions for assay validation. For sensitivity and specificity, radiolabeled known drugs are used in competitive binding assays. The assay is designed as a competitive inhibition assay using the radiolabeled known drug/ligand receptor interaction to screen chemical or natural product libraries for more effective NCEs (new chemical entities).

These quantitative binding parameter determinations indicate the minimal effective drug concentrations. MultiScreen_HTS plates for quantitative binding parameters provide a more accurate and reliable alternative to homogeneous assays. They are widely used in high throughput screening campaigns and provide a reliable platform that incorporates a range of glass fiber filters to retain the receptor and “bound” ligand fraction. Operation by vacuum manifold allows for more convenient characterizations since the bound fractions are easily collected from the top of the plate.

The improved plate design of MultiScreen_HTS automation-compatible
filter plates facilitates use with gripper arms and is compatible with microplate scintillation counters.

MultiScreen_HTS+ Hi Flow filter plates: improve washing efficiency and reduce data variation.

MultiScreen Harvest plates are also available to withstand the numerous washes and batch pretreatment (PEI) required in cell harvest protocols.

Highly sensitive and specific radiometric assays

Compatible with liquid scintillation cocktail
Improved automation compatibility with new MultiScreen_HTS filter plates

Protocol

1.	Add cells, membrane, or tissue to MultiScreen Plate
2.	Incubate with labeled ligand
3.	Collect receptor/ligand complex on filter membrane. Wash and count. If "free vs. bound" study, collect filtrate into 96-well plate

Performance

Optimized Design for High Quality Results

384-well Displacement Binding of Human Muscarinic M1 Receptor

96-well Displacement Binding of Human Muscarinic M1 Receptor

Radioligand binding displacement binding assays were performed with a constant radiolabeled scopolamine concentration (0.6 nM) and serial dilutions of unlabelled pirenzipine as compared to a control binding experiment without unlabelled pirenzipine (% of Control). Left: Displacement binding done with 4.38 µg receptor preparation in 100 µL using MutliScreen_HTS 384-well filter plate. Results presented are from three separate experiments each performed in triplicate wells. Right: Displacement binding done with 8.75 µg receptor preparation in 200 µL using MutliScreen 96-well filter plate. Relative affinity values (IC50) were determined by fitting displacement binding inhibition values by non-linear regression using Prism™ data software. All data points are the average of triplicate experiments. Filter plates were dried prior to the addition of Supermix™ (10 µL in 384-well plates and 50 µL in 96-well plates). Counting was done in a MicroBeta® Trilux. NOTE: All counting is done with underdrain on for both 96- and 384-well plates.

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