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14-451M ROKα/ROCK-II Protein, active, 250 µg

Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.

ROKα/ROCK-II Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-451M
250 µg  Also available in 10 μg size (cat#14-451) and in bulk (cat# 14-451-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-451M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionROKα/ROCK-II Protein, active, 250 µg
OverviewN-terminal His6-tagged recombinant human ROCK-II residues 11-552
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Gene Symbol
  • ROCK1
  • P160ROCK
  • MGC131603
  • p160ROCK
  • MGC43611
  • p160-ROCK
Protein TargetROKα/ROCK-II
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13464 # Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere- dependent exit from mitosis (By similarity).
SIZE: 1354 amino acids; 158175 Da
SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM (By similarity). Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2.
SUBCELLULAR LOCATION: Cytoplasm. Centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.
TISSUE SPECIFICITY: Detected in blood platelets.DOMAIN:SwissProt: Q13464 The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. & Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 REM (Hr1) repeat.
MISCELLANEOUS: Inhibited by Y-27632.
Molecular Weight63.3kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-451) and in bulk (cat# 14-451-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-451M 04053252673061

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-451 ROKα/ROCK-II Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases