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524400 Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem

Phorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PMA, TPA, PKC Activator I

Primary Target: Protein Kinase C
524400
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
16561-29-8C₃₆H₅₆O₈

Products

Catalogue NumberPackaging Qty/Pack
524400-10MG Glass bottle 10 mg
524400-1MG Glass bottle 1 mg
524400-25MG Glass bottle 25 mg
524400-5MG Glass bottle 5 mg
Description
OverviewMost commonly-used phorbol ester. Extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro, even at nM concentrations. Promotes the expression of inducible NOS in cultured hepatocytes. Activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Its binding is reversible. Also available as a 10 mM solution in DMSO (Cat. No. 500582).
Catalogue Number524400
Brand Family Calbiochem®
SynonymsPMA, TPA, PKC Activator I
References
ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
Perchellet, J. 1985. Carcinogenesis 6, 567.
Nishizuka, Y. 1984. Science 255, 1365.
Mastro, A. 1982. Lymphokines 6, 263.
Product Information
CAS number16561-29-8
ATP CompetitiveN
FormWhite solid
Hill FormulaC₃₆H₅₆O₈
Chemical formulaC₃₆H₅₆O₈
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationPhorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.
Biological Information
Primary TargetProtein Kinase C
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSQH4377000
Safety Information
R PhraseR: 21-38

Harmful in contact with skin.
Irritating to skin.
S PhraseS: 26-27-36-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
524400-10MG 04055977270952
524400-1MG 04055977270945
524400-25MG 04055977270969
524400-5MG 04055977270976

Documentation

Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem Certificates of Analysis

TitleLot Number
524400

References

Reference overview
Powell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
Perchellet, J. 1985. Carcinogenesis 6, 567.
Nishizuka, Y. 1984. Science 255, 1365.
Mastro, A. 1982. Lymphokines 6, 263.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Cvetanovic, M., et al. 2006. Journal of Biological Chemistry 281, 20055.
  • Jafri, F., et al. 2006. Journal of Biological Chemistry 281, 19346.
  • Jiang, X., et al. 2005. Arteriosclerosis, Thrombosis, and Vascular Biology 25, 2515.
  • Yoon-Jin Lee, et al. 2005. Journal of Biological Chemistry 280, 18108.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision19-October-2010 JSW
    SynonymsPMA, TPA, PKC Activator I
    DescriptionExtremely potent mouse skin tumor promoter. Activates protein kinase C (PKC) in vivo and in vitro, even at nanomolar concentrations. Promotes the expression of iNOS in cultured hepatocytes. Activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Most commonly used phorbol ester.
    FormWhite solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number16561-29-8
    RTECSQH4377000
    Chemical formulaC₃₆H₅₆O₈
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (10 mg/ml). Further dilute with aqueous buffers just prior to use.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
    Toxicity Harmful
    ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
    Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
    Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
    Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
    Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
    Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
    Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
    Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
    Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
    Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
    Perchellet, J. 1985. Carcinogenesis 6, 567.
    Nishizuka, Y. 1984. Science 255, 1365.
    Mastro, A. 1982. Lymphokines 6, 263.
    Citation
  • Cvetanovic, M., et al. 2006. Journal of Biological Chemistry 281, 20055.
  • Jafri, F., et al. 2006. Journal of Biological Chemistry 281, 19346.
  • Jiang, X., et al. 2005. Arteriosclerosis, Thrombosis, and Vascular Biology 25, 2515.
  • Yoon-Jin Lee, et al. 2005. Journal of Biological Chemistry 280, 18108.
    		•