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499260 Osteopontin, His•Tag®, Mouse, Recombinant, Mouse

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rmOPN

499260
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
499260-50UG Plastic ampoule 50 μg
Description
OverviewRecombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
M.W. 65,000 and 30,000 under reducing conditions.
Catalogue Number499260
Brand Family Calbiochem®
SynonymsrmOPN
References
ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
Quality LevelMQ100
Applications
Biological Information
Biological activityED50 = 0.25-1.5 µg/ml
based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxins: ≤100 pg/µg rmOPN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
499260-50UG 04055977199918

Documentation

Osteopontin, His•Tag®, Mouse, Recombinant, Mouse SDS

Title

Safety Data Sheet (SDS) 

Osteopontin, His•Tag®, Mouse, Recombinant, Mouse Certificates of Analysis

TitleLot Number
499260

References

Reference overview
Xie, Y., et al. 2001. Kidney Int. 60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-June-2019 JSW
SynonymsrmOPN
DescriptionRecombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
FormLyophilized
FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxins: ≤100 pg/µg rmOPN
Biological activityED50 = 0.25-1.5 µg/ml
based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
SolubilityReconstitute to ≥100 µg/ml with sterile PBS.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
Toxicity Standard Handling
ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.