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APT242 MitoLight® Mitochondrial Apoptosis Detection Kit

The MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Flow Cytometry
APT242
100 assays  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsDetection Methods
FCFluorescent
Description
Catalogue NumberAPT242
Brand Family Chemicon®
Trade Name
  • MitoLight
  • Chemicon
DescriptionMitoLight® Mitochondrial Apoptosis Detection Kit
OverviewDisruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.

MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).
Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

· 37°C CO2 incubator

· Flow Cytometer

· Glass slides with glass coverslips
Product Information
Components
  • MitoLight™ (Part No. 71616): 100 μL in DMSO.
  • 10X Incubation Buffer (Part No. 71614): 20 mL.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationThe MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.
Key Applications
  • Flow Cytometry
Biological Information
Species Reactivity
  • Mammals
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore kit materials at -20°C up to their expiration date. After thawing, unused kit components can be refrozen. For best results, aliquot the MitoLight™ reagent into separate vials to avoid repeated freeze/thaw cycles.

PROTECT REAGENTS FROM LIGHT.
Packaging Information
Material Size100 assays
Global Trade Item Number
Catalogue Number GTIN
APT242 08436037125430