Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

HSTCMAG28SPMX21 MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease
HSTCMAG28SPMX21
96-well plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α HMplexLuminex xMAP
Description
Catalogue NumberHSTCMAG28SPMX21
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. These features are summarized on the next page.
• This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Fractalkine
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-21
  • IL-23
  • ITAC
  • MIP-1α
  • MIP-1β
  • MIP-3α
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 98% – 107%
Physicochemical Information
Sensitivity
  • 0.11 - 8.17 pg/mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • Fractalkine: 18.3 – 75,000 pg/mL
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • IL-17A: 0.73 – 3,000 pg/mL
  • IL-21: 0.24 – 1,000 pg/mL
  • IL-23: 7.93 – 32,500 pg/mL
  • ITAC: 1.46 – 6,000 pg/mL
  • MIP-1α: 0.31 – 1,250 pg/mL
  • MIP-1β: 0.92 – 3,750 pg/mL
  • MIP-3α: 0.61 – 2,500 pg/mL
  • TNFα: 0.45 – 1,750 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HSTCMAG28SPMX21 04055977289114

Documentation

Protocols

Title
Protocol- HSTCMAG-28SK|HSTCMAG28SPMX13|HSTCMAG28SPMX21|HSTCMAG28PMX13BK|HSTCMAG28PMX21BK

MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
MILLIPLEX MAP Human High Sensitivity T Cell Panel
Quality Control Range Sheet- HST-111 and HST-211
Quality Control Ranges: Milliplex Human High Sensitivity T Cell Magnetic Bead Panel