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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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508228
Sigma-AldrichL- Buthionine Sulfoximine - CAS 83730-53-4 - Calbiochem
A cell-permeable, sulfoximine based compound that acts a potent, fast acting, irreversible inhibitor of g-glutamylcysteine synthetase and depletes cellular glutathione levels. Closely resembles the structure of g-glutamylphosphate cysteine adduct. The inhibition follows the first order kinetics with t1/2 = ~ 11 sec at saturating inhibitor concentration. Does not affect the activity of glutamine synthetase. Shown to improve the outcome of chemotherapy by lowering the levels of glutathione in cancer cells (IC50 = 1.9, 8.6, and 29 µM in melanoma, breast, and ovarian tumor cells, respectively).
Catalogue Number
508228
Brand Family
Calbiochem®
References
References
Fruehauf, J.P., et al. 1997. Pigment Cell Res.10, 236. OʹDwyer, P.J., et al. 1996. J. Clin. Oncol.14, 249. Griffith, O. W., 1982. J. Biol. Chem.257, 13704. Griffith, O. W., et al. 1979. J. Biol. Chem.254, 7558.
Fruehauf, J.P., et al. 1997. Pigment Cell Res.10, 236. OʹDwyer, P.J., et al. 1996. J. Clin. Oncol.14, 249. Griffith, O. W., 1982. J. Biol. Chem.257, 13704. Griffith, O. W., et al. 1979. J. Biol. Chem.254, 7558.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
31-October-2013 JSW
Description
A cell-permeable, sulfoximine based compound that acts a potent, fast acting, irreversible inhibitor of g-glutamylcysteine synthetase and depletes cellular glutathione levels. Closely resembles the structure of g-glutamylphosphate cysteine adduct. The inhibition follows the first order kinetics with t1/2 = ~ 11 sec at saturating inhibitor concentration. Does not affect the activity of glutamine synthetase. Shown to improve the outcome of chemotherapy by lowering the levels of glutathione in cancer cells (IC50 = 1.9, 8.6, and 29 µM in melanoma, breast, and ovarian tumor cells, respectively).
Form
White powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
83730-53-4
Chemical formula
C₈H₁₈N₂O₃S
Structure formula
Purity
≥97% by TLC
Solubility
H₂O (50 mg/ml)
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Fruehauf, J.P., et al. 1997. Pigment Cell Res.10, 236. OʹDwyer, P.J., et al. 1996. J. Clin. Oncol.14, 249. Griffith, O. W., 1982. J. Biol. Chem.257, 13704. Griffith, O. W., et al. 1979. J. Biol. Chem.254, 7558.
