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14-1057 Human native nucleosomes

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.

Human native nucleosomes MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Activity Assay
14-1057
20 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
ACT
Description
Catalogue Number14-1057
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHuman native nucleosomes
OverviewHuman native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.
Background InformationNucleosomes are the basic units of chromatin, and are comprised of approximately 147 bp of DNA spooled around a central octamer of core histones, H2A, H2B, H3, and H4. The arrangement of nucleosomes in chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of the core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
References
Product Information
Presentation20 μg of mononucleosomes in buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 0.5 mM PMSF,1 mM DTT, and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationHuman native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.
Key Applications
  • Activity Assay
Application NotesSuitable for in vitro chromatin assembly and enzyme activity assays
Biological Information
Concentration0.2 µg/µL
Purity>98% purity by SDS PAGE
SourceHuman
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at -80ºC for 1 year from date of receipt.
Packaging Information
Material Size20 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-1057 04053252694653

Documentation

Human native nucleosomes SDS

Title

Safety Data Sheet (SDS) 

Human native nucleosomes Certificates of Analysis

TitleLot Number
Human native nucleosomes - 2276397 2276397
Human native nucleosomes - 2527355 2527355
Human native nucleosomes - 2987717 2987717
Human native nucleosomes - 3174013 3174013
Human native nucleosomes - 3212332 3212332
Human native nucleosomes - 3253395 3253395
Human native nucleosomes - 3471938 3471938
Human native nucleosomes - 3506084 3506084
Human native nucleosomes - 3560695 3560695
Human native nucleosomes - 3593887 3593887
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