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HPI-15K Human Proinsulin RIA

HPI-15K
250 tubes  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Proinsulin HRIARadioactive 125I
Description
Catalogue NumberHPI-15K
DescriptionHuman Proinsulin RIA
Background InformationProinsulin is made by the beta cells of the pancreas
Product Information
Detection methodRadioactive 125I
Performance
  • ED80 = 10 ± 1 pM
  • ED50 = 23 ± 2 pM
  • ED20 = 52 ± 3 pM
Precision, %
  • Inter-assay: 1.5–10.1
  • Intra-assay: 1.5–6.9
Quality LevelMQ100
Applications
ApplicationThis radioimmunoassay (RIA) measures & quantifies Proinsulin for use in Metabolism & Endocrine research.
Key Applications
  • Radioimmunoassay
Application NotesThree-day, disequilibrium incubation at room temperature. Sample volume ≤200 µL serum, plasma, or tissue culture media.
Biological Information
SpecificityIntact Human Proinsulin 100% Des 31, 32 HPI 95% Des 64, 65 HPI <0.1% Bovine, Porcine Proinsulin Not Detectable Human Insulin <0.1% Human C-Peptide <0.1% Glucagon Not Detectable Somatostatin, PP Not Detectable Human IGF-I Not Detectable Human IGF
Species Reactivity
  • Human
Analytes Available
  • Proinsulin
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
Radioactive Isotype125I-Human Proinsulin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 91–93%
Physicochemical Information
Linearity of Dilution100–120%
Standard Curve Range
  • 2–100 pM
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size250 tubes
Global Trade Item Number
Catalogue Number GTIN
HPI-15K 04053252319181