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14-843M Hck Protein, activated, 250 µg

Activated, N-terminal 6His-tagged, recombinant, human Hck, amino acids 230–497, for use in Kinase Assays.

14-843M
250 µg  Also available in 10 μg size (cat#14-843) and in bulk (cat# 14-843-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-843M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHck Protein, activated, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human Hck, amino acids 230–497
References
Product Information
Presentation50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.
Quality LevelMQ300
Applications
ApplicationActivated, N-terminal 6His-tagged, recombinant, human Hck, amino acids 230–497, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Application NotesAuto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
Gene Symbol
  • HCK
  • p59-HCK/p60-HCK
  • JTK9
Protein TargetHck
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08631 # May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
SIZE: 526 amino acids; 59600 Da
SUBUNIT: May bind to HIV-1 Nef and VIF through its SH3 domain. This interaction would stimulates its tyrosine-kinase activity.
SUBCELLULAR LOCATION: Isoform p59-HCK: Membrane; Lipid-anchor. & Isoform p60-HCK: Membrane; Lipid-anchor. Cytoplasm.
TISSUE SPECIFICITY: Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
DOMAIN: SwissProt: P08631 The SH3 domain mediates binding to HIV-1 Nef.
PTM: Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3 (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight34.1kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-843) and in bulk (cat# 14-843-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-843M 04053252282737

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-843 Hck Protein, activated, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases