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324726 Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli

324726
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Overview

Products

Catalogue NumberPackaging Qty/Pack
324726-200MIU Plastic ampoule 200 miu
Description
OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
Note: 1 mU = 1 milliunit.
Catalogue Number324726
Brand Family Calbiochem®
SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
References
ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Product Information
Activity≥5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
EC number3.2.1.96
FormLiquid
FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
Quality LevelMQ100
Biological Information
Specific Activity≥20 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
324726-200MIU 04055977216004