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324682 Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
324682
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
324682-1000U Plastic ampoule 1000 u
324682-250U Plastic ampoule 250 u
Description
OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
Catalogue Number324682
Brand Family Calbiochem®
References
ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
Mandl, I. 1962. Methods in Enzymol. 5, 665.
Product Information
CAS number39445-21-1
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
EC number3.4.21.36
FormLyophilized
FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥25 units/mg protein
Physicochemical Information
ContaminantsChymotrypsin: ≤0.2%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSTS5052000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
324682-1000U 04055977196832
324682-250U 07790788054380

Documentation

Elastase, Porcine Pancreas, High Purity, Crystallized SDS

Title

Safety Data Sheet (SDS) 

Elastase, Porcine Pancreas, High Purity, Crystallized Certificates of Analysis

TitleLot Number
324682

References

Reference overview
Largman, C. et al. 1976. Biochemestry 15, 2491.
Mandl, I. 1962. Methods in Enzymol. 5, 665.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-July-2010 JSW
DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
FormLyophilized
FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
CAS number39445-21-1
RTECSTS5052000
EC number3.4.21.36
ContaminantsChymotrypsin: ≤0.2%
Specific activity≥25 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
SolubilitydH₂O (10 mg/ml)
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
Toxicity Standard Handling
Merck USA index14, 3535
ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
Mandl, I. 1962. Methods in Enzymol. 5, 665.

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Categories

Life Science Research > Proteins and Enzymes > Other Enzymes