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328005 ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem

The ERK Activation Inhibitor Peptide II, Cell-Permeable controls the biological activity of ERK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MTPTAT-G-MEK1₁₃, H-GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH₂, Erk Inhibitor V

Primary Target: MEK
328005
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₃₆H₂₄₁N₅₃O₃₁S

Products

Catalogue NumberPackaging Qty/Pack
328005-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that is fused to the HIV-TAT membrane translocating peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 210 nM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC50 = 29 µM). Does not affect the activation of JNKs or p38.
Catalogue Number328005
Brand Family Calbiochem®
SynonymsMTPTAT-G-MEK1₁₃, H-GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH₂, Erk Inhibitor V
References
ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
Product Information
ATP CompetitiveN
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₃₆H₂₄₁N₅₃O₃₁S
Chemical formulaC₁₃₆H₂₄₁N₅₃O₃₁S
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetMEK
Primary Target IC<sub>50</sub>210 nM against MEK; 29 µM in blocking ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
328005-1MG 04055977196344

Documentation

ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
328005

References

Reference overview
Kelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-January-2010 RFH
SynonymsMTPTAT-G-MEK1₁₃, H-GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH₂, Erk Inhibitor V
DescriptionA cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK or MAPKK) that is fused to the HIV-TAT membrane translocation peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation by MEK1 and the downstream transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 210 nM in vitro). Potently blocks ERK in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC50 = 29 µM). Does not inhibit the activation of c-jun N-terminal kinases (JNKs) or p38 MAP kinase.
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₃₆H₂₄₁N₅₃O₃₁S
Peptide SequenceH-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂
Purity≥95% by HPLC
SolubilityH₂O (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.