Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

100591 ELASTIN staining solution acc. to Weigert

for microscopy

ELASTIN staining solution acc. to Weigert MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
100591
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
1005910500 Plastic bottle 500 ml
Add To Favorites
Description
Catalogue Number100591
DescriptionELASTIN staining solution acc. to Weigert
OverviewThe ELASTIN staining solution acc. to Weigert - for microscopy, is a ready-to-use solution used to detect elastic fibers in connective tissues. Elastic connective-tissue fibers are bundles of glycoprotein-containing microfibrils that accumulate the polymeric protein elastin in their development, gradually resulting in the formation of coarse elastic fibers and membranes. The microfibrils on the surface of the elastic connective-tissue fibers expose high numbers of disulfide groups, which can be chemically detected when sufficient material is available for light-microscopic investigations. Furthermore, they are strongly acidic and thus bind the resorcin-fuchsin dye by electropolarity via interface adsorption.
The resorcin-fuchsin staining method is a regressive method. In tissue sections, nuclei can be stained using a 0.1% nuclear fast red-aluminum sulfate solution (Cat.no. 100121) if not only elastic fibers, but also nuclei and the background also need to be visualized.
Brilliant staining results of these fibres are usually achieved when using the ELASTIN staining solution acc. to Weigert - for microscopy in combination with the Picrofuchsin solution acc. van Gieson - for microscopy, Cat. No. 100199 and nuclear staining with Weigert’s iron hematoxylin kit - for nuclear staining in histology, Cat. No. 115973 respectively Nuclear fast red-aluminium sulfate solution 0.1% - for microscopy, Cat. No. 100121.
The 500ml bottle is sufficient for 400 - 500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Product Information
HS Code3212 90 00
Quality LevelMQ400
Applications
Applicationfor microscopy
Physicochemical Information
Boiling point78.3 °C (1013 hPa) (ethanol)
Density0.84 g/cm3 (20 °C)
Explosion limit3.1 - 27.7 %(V) (ethanol)
Flash point18 °C
Vapor pressure59 hPa (20 °C)
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H290: May be corrosive to metals.
H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
H351: Suspected of causing cancer.
H371: May cause damage to organs.
Precautionary Statement(s)P202: Do not handle until all safety precautions have been read and understood.
P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308 + P311: IF exposed or concerned: Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 3 highly hazardous to water
Safety Information
Categories of dangerhighly flammable
Storage and Shipping Information
StorageStore at +2°C to +8°C.
Transport Information
Declaration (railroad and road) ADR, RIDUN 2924 , 3 (8), II
Declaration (transport by air) IATA-DGRUN 2924 , 3 (8), II
Declaration (transport by sea) IMDG-CodeUN 2924 , 3 (8), II, Segregation Group: 1 (Acids)
Specifications
Suitability for microscopypasses test
Nucleiblue-brown
Collagenred
Muscle fibersyellow
Cytoplasmyellow
Elastic fibersblack
Global Trade Item Number
Catalogue Number GTIN
1005910500 04027269377861