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17-603 Sigma-AldrichChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set

This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

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Key Applications: Western Blotting, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology

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Key Spec Table

Species Reactivity	Key Applications
H, M	WB, ChIP

Description
Catalogue Number	17-603
Brand Family	Upstate
Trade Name	ChIPAb+ Upstate
Description	ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set
Overview	Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. ERα antibody is functionally validated in the precipitation of ERα associated chromatin. The qPCR primers included flank the ERα binding site in human pS2 promoter.

References

Product Information
Format	Ascites
Control	Includes control primers specific for human TFF1 (pS2) promoter.
Presentation	Anti-ERα (mouse ascites). 1 vial containing 100 mL ascites. Store at -20°C. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα. ChIP primers TFF1 (pS2). 1 vial containing 75 mL of 5 μM of each control primer specific for human TFF1 (pS2) promoter. Store at -20°C. FOR: CCG GCC ATC TCT CAC TAT GAA REV: CCT TCC CGC CAG GGT AAA TAC
Quality Level	MQ100

Applications
Application	This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	Western Blotting Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation: Sonicated chromatin, prepared from 3x10⁶ MCF7 cells that are either estrogen starved or b-estradiol treated (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERa and the Magna ChIP™ G (Cat.# 17-611) kit Standard Protocol. Successful enrichment of ER associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details. Western Blot Analysis: MCF7cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-ERa (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

Biological Information
Immunogen	The ERα antibody is made against aa 120-170 of bovine estrogen α.
Host	Mouse
Specificity	Estrogen Receptor α
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000125.2
Entrez Gene Summary	This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
Gene Symbol	ESR1 Era ER-alpha ESR ER NR3A1 ESRA DKFZp686N23123
UniProt Number	P03372
UniProt Summary	FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
Molecular Weight	66 kDa

Physicochemical Information

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Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Chromatin immunoprecipitation: Sonicated chromatin prepared from 3x10⁶ MCF7 cells that were treated with b-estradiol (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERα and beads only control. Successful enrichment of ERα associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat.# 17- 409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Chromatin immunoprecipitation:
Sonicated chromatin prepared from 3x10⁶ MCF7 cells that were treated with b-estradiol (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERα and beads only control.
Successful enrichment of ERα associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat.# 17- 409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	1 year at -20°C from date of shipment

Packaging Information
Material Size	25 assays
Material Package	25 assays per kit. ~4 μL per chromatin immunoprecipitation.

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
17-603	04053252516146

Documentation

ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set SDS

Title
Safety Data Sheet (SDS)

ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set Certificates of Analysis

Title	Lot Number
ChIPAb+ Estrogen Receptor - 2225263	2225263
ChIPAb+ Estrogen Receptor #945; - 2113337	2113337
ChIPAb+ Estrogen Receptor - R0708G0052	R0708G0052
ChIPAb+ Estrogen Receptor a - 2155400	2155400
ChIPAb+ Estrogen Receptor a - DAM1480392	DAM1480392
ChIPAb+ Estrogen Receptor a - DAM1764569	DAM1764569
ChIPAb+ Estrogen Receptor a - JBC1862628	JBC1862628
ChIPAb+ Estrogen Receptor a - JBC1940026	JBC1940026
ChIPAb+ Estrogen Receptor a - JBC1941197	JBC1941197
ChIPAb+ Estrogen Receptor α - 3185805	3185805

Brochure

Title
Advance your Epigenetics Research
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

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