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69968 Biotinylated T7•Tag® Monoclonal Antibody

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69968
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69968-3 Plastic ampoule 125 ul
Description
Overview

This product has been discontinued.





When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs.

The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.

SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)
FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69968
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69968-3 04055977274080

Documentation

Biotinylated T7•Tag® Monoclonal Antibody Certificates of Analysis

TitleLot Number
69968

Citations

Title
  • Hiroko Hayakawa, et al. (2007) Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation. Journal of Biological Chemistry 282, 26369-26380.
  • Paul H. Bessette, Jeffrey J. Rice and Patrick S. Daugherty. (2004) Rapid isolation of high-affinity protein binding peptides using bacterial display. Protein Engineering Design and Selection 17, 731-739.
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    User Protocols

    Title
    TB106 Biotinylated T7•Tag® Monoclonal Antibody