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72158 BacMagic™-2 Transfection Kit - Novagen

BacMagic™-2 Transfection Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
72158
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
72158-3 Plastic ampoule 5 rxn
Description
OverviewThe BacMagic™-2 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic™ DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 Transfection Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
Not available for sale in Japan
Catalogue Number72158
Brand Family Novagen®
Application Data
Figure 1. Construction of baculovirus recombinants with three BacMagic systems

Figure 2. Streamlined baculovirus method
References
Product Information
•0.5 µgBacMagic-2 DNA
•50 µlInsect GeneJuice Transfection Reagent
•2 x 1 mlSf9 Cells
•1 LBacVector® Insect Cell Medium
•2 µgTransfection Control Plasmid
DeclarationNot available for sale in Japan.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 24/25-26-36/37/39

Avoid contact with skin and eyes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
Product Usage Statements
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
72158-3 04055977266481

Documentation

BacMagic™-2 Transfection Kit - Novagen Certificates of Analysis

TitleLot Number
72158

User Protocols

Title
TB459 BacMagic™ DNA Kits