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07-449-AF555 Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody

This Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
07-449-AF555
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®555Polyclonal Antibody
Description
Catalogue Number07-449-AF555
DescriptionAnti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody
Alternate Names
  • Histone H3.1t
  • H3/t
  • H3t
  • H3/g
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
Product Information
FormatAlexaFluor®555
PresentationPurified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenBranch peptide corresponding to trimethyl-Histone H3 (Lys27).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThe unconjugated parent antibody of this antibody is Cat. No. 07-449.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~17 kDa observed. The unconjugated parent antibody (Cat. No. 07-449) has an observed molecular weight at ~17 kDa.
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
07-449-AF555 04053252983689