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05-900 Anti-phospho-Met (Tyr1234/1235) Antibody, clone NL8, rabbit monoclonal

05-900
100 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedMonoclonal Antibody
Description
Catalogue Number05-900
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Met (Tyr1234/1235) Antibody, clone NL8, rabbit monoclonal
Alternate Names
  • c-Met
  • cmet
  • HGFR
  • RCCP2
  • met proto-oncogene (hepatocyte growth factor receptor)
References
Product Information
FormatPurified
Applications
ApplicationAnti-phospho-Met (Tyr1234/1235) Antibody, clone NL8 detects level of phospho-Met (Tyr1234/1235) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (...KE[pY][pY}SV...) in which pYpY corresponds to phosphorylated amino acids 1234 and 1235 of human Met/HGFR
CloneNL8
HostRabbit
Specificityphosphorylated Met, cross-reacts with phosphorylated EGFR
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteBroad species cross-reactivity expected due to sequence homology
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
Gene Symbol
  • MET
  • HGFR
  • AUTS9
  • c-Met
  • RCCP2
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
Molecular WeightMr 140kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from NIH-3T3 cells co-expressing Met/HGFR and HGF
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditionsstable 2 years at -20°C from date of shipment
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-900 04053252366390

Documentation

Anti-phospho-Met (Tyr1234/1235) Antibody, clone NL8, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-Met (Tyr1234/1235), clone NL8 - 30368 30368

References

Reference overviewPub Med ID
Has HGF met other partners? Met-independent epithelial morphogenesis induced by HGF. focus on "Hepatocyte growth factor induces MDCK cell morphogenesis without causing loss of tight junction functional integrity".
Devarajan, Prasad
Am. J. Physiol., Cell Physiol., 286: C475-7 (2004)  2004

14761880 14761880
Therapeutic targeting of the receptor tyrosine kinase Met.
Sattler, Martin, et al.
Cancer Treat. Res., 119: 121-38 (2004)  2004

15164876 15164876
Prostate cancer and the met hepatocyte growth factor receptor.
Knudsen, Beatrice S and Edlund, Magnus
Adv. Cancer Res., 91: 31-67 (2004)  2004

Show Abstract
15327888 15327888
c-Met: structure, functions and potential for therapeutic inhibition.
Ma, Patrick C, et al.
Cancer Metastasis Rev., 22: 309-25 (2003)  2003

Show Abstract
12884908 12884908
Met, metastasis, motility and more.
Birchmeier, Carmen, et al.
Nat. Rev. Mol. Cell Biol., 4: 915-25 (2003)  2003

14685170 14685170