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MABS494 Anti-phospho MEKK5, (Thr845), clone PA214 Antibody

Anti-phospho MEKK5, (Thr845), clone PA214 | MABS494 is an antibody against phospho MEKK5 for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: MAP3K5, ASK1, MAPKKK5, MEKK5
UniProt Number: Q99683
MABS494
200 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS494
DescriptionAnti-phospho MEKK5, (Thr845), clone PA214 Antibody
Alternate Names
  • MEKK5
  • Apoptosis signal-regulating kinase 1
  • MAPK/ERK kinase kinase 5
  • ASK-1
  • MEK kinase 5
  • MEKK 5
  • ASK1
Background InformationMEKK5 (MAP3K5) is a key kinase for our innate immunity and for mediating cellular signaling cascades evoked by changes in the external cellular environment. As such, MEKK5 is critical for cell fate during development, apoptosis, and cell survival as well as in modulating the host defense responses from a wide range of pathogens. MEKK5 acts as an upstream activator of the MKK/JNK signaling pathways and the p38 MAPK signaling pathways that ultimately results in activating multiple kinases such as MAP2K3/Mkk3 or MKK6/MMK7. Activation of MMEKK5 is via phosphorylation of Thr-845 within the activation loop via the recently discovered kinase MAP3K6 which forms a complex with MEKK5 and also stabilizes the protein. Expression is widespread and localized in the cytoplasmic in the perinuclear endoplasmic reticulum region.
References
Product Information
FormatPurified
PresentationPurified rabbit monoclonal supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho MEKK5, (Thr845), clone PA214 | MABS494 is an antibody against phospho MEKK5 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
Immunogenlinear peptide corresponding to Human phospho MEKK5 (Thr 845).
ClonePA214
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAP3K5
  • ASK1
  • MAPKKK5
  • MEKK5
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~160 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on HEK293A lysate
Western Blotting Analysis: 4 µg/mL dilution of this antibody detected phosphorylated MEKK5 protein in 10 µg of H2O2 treated Hek293A cell lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS494 04055977123883