Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

05-806-AF647 Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 Antibody

This Anti-phospho Histone H3 (Ser10) antibody, clone 3H10, Alexa Fluor® 647 is a purified mouse monoclonal for detection of Histone H3 phosphorylated at serine 10.
This mAb is fluorescently labeled and validated for use in IC. The unconjugated version has been proven in FC, ICC, IF, PIA, WB, Mplex.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695
05-806-AF647
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-806-AF647
DescriptionAnti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 Antibody
Alternate Names
  • H3S10P
  • Histone H3 (phospho Ser10)
  • H3 histone, family 3A
  • H3.3A
  • H3 histone, family 3B
  • H3.3B
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 05-806.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse conjugated antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H3 (Ser10) antibody, clone 3H10, Alexa Fluor® 647 is a purified mouse monoclonal for detection of Histone H3 phosphorylated at serine 10.
This mAb is fluorescently labeled and validated for use in IC. The unconjugated version has been proven in FC, ICC, IF, PIA, WB, Mplex.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenLinear peptide corresponding to Histone H3 phosphorylated at Ser10.
Clone3H10
HostMouse
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
  • HIST3H3
  • H3FT
  • H3T
  • MGC126886
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed (Refer to Cat. No. 05-806)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry Analysis in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-806-AF647 04053252971570

Documentation

Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 Antibody Certificates of Analysis

TitleLot Number
Anti-phospho Histone H3 (Ser10), -2813496 2813496
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - Q2418798 Q2418798
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 3273617 3273617
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 3462126 3462126
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 3704777 3704777
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 3934349 3934349
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 4048032 4048032
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 - 4154507 4154507
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 -2534862 2534862
Anti-phospho Histone H3 (Ser10), clone 3H10, Alexa Fluor® 647 -2673163 2673163
Show more

Related Products & Applications

Product Families

Conjugated Antibodies for Chromatin Analysis

EMD Millipore's Alexa Fluor® Conjugated Histone and puromycin antibodies are validated in immunocytochemistry. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon.
Learn More >>

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies