Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

06-942-S Sigma-AldrichAnti-acetyl-Histone H3 (Lys9) Antibody, Trial Size

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9 (H3K9ac). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ChIP, ChIP-seq, FC. Small pack size is ideal for screening applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695

Recommended Products

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Eu	DB, WB, ChIP-seq	Rb	Purified	Polyclonal Antibody

Description
Catalogue Number	06-942-S
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size
Alternate Names	H3K9Ac Histone H3 (acetyl K9) H3 histone family, member T Histone 3, H3 Histone cluster 3, H3
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

References

Product Information
Format	Purified
Control	TSA-treated HeLa and NIH/3T3 cells.
Presentation	Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Quality Level	MQ100

Applications
Application	Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9 (H3K9ac). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ChIP, ChIP-seq, FC. Small pack size is ideal for screening applications.
Key Applications	Dot Blot Western Blotting ChIP-seq
Application Notes	Western Blot Analysis: A431, C2C12 lysate, HEK293 lysate, Hela lysate, HepG2 lysate , Jurkat lysate , L6 lysate, and NIH/3T3 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ANTI-ACETYL HIS H3 (1:500 dilution of a representative lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 06-942-S, Anti-acetyl-Histone H3 (Lys9) at a (1:500) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Dot Blot Analysis: 0.5 μg/mL of a previous lot detected 50 ng of mono-acetylated peptide corresponding to position 9 in the N-terminal sequence of Histone H3 (Catalog # 12-358), but not the monoacetylated peptide corresponding to acetyl-lysine at position 14 (Catalog # 12-359) or unacetylated Histone H3 (Catalog # 12-357). Immunoprecipitation: Not determined. Immunocytochemistry: Not determined.

Biological Information
Immunogen	KLH-conjugated, synthetic peptide (ARTKQTA RAcKSTG-C) corresponding to amino acids 1-12 of Histone H3.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Histone H3 acetylated at position 9.
Isotype	IgG
Species Reactivity	Human Mouse Rat Eukaryote
Species Reactivity Note	Human, although this peptide sequence is identical in a wide range of animal and plant species.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_003493
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol	HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T MGC126886
Modifications	Acetylation
Purification Method	Protein A Purfied
UniProt Number	Q16695
UniProt Summary	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Molecular Weight	Approx. 17 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on HEK293 lysates. Western Blot Analysis: 1:500 dilution of this lot detected ANTI-ACETYL HIS H3 on 10 μg of HEK293 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	20 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
06-942-S	04053252941009

Documentation

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size SDS

Title
Safety Data Sheet (SDS)

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size Certificates of Analysis

Title	Lot Number
Anti-acetyl-Histone H3 (Lys9), -2514256	2514256
Anti-acetyl-Histone H3 (Lys9), -2608852	2608852
Anti-acetyl-Histone H3 (Lys9), -2624583	2624583
Anti-acetyl-Histone H3 (Lys9), -2643625	2643625
Anti-acetyl-Histone H3 (Lys9), -2702107	2702107
Anti-acetyl-Histone H3 (Lys9), -2795417	2795417
Anti-acetyl-Histone H3 (Lys9), Trial Size - 3599020	3599020
Anti-acetyl-Histone H3 (Lys9), Trial Size - 3861837	3861837
Anti-acetyl-Histone H3 (Lys9), Trial Size - 3992253	3992253
Anti-acetyl-Histone H3 (Lys9), Trial Size - Q2343956	Q2343956

The Product Has Been Added To Your Cart