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MABD34 Sigma-AldrichAnti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1

Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: WRN, RECQ3, RECQL2 UniProt Number: Q14191

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABD34
Description	Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1
Alternate Names	Werner syndrome ATP-dependent helicase DNA helicase, RecQ-like type 3 RecQ3 Exonuclease WRN RecQ protein-like 2
Background Information	The Werner’s syndrome helicase (RecQ-like type 3; RecQ3; Exonuclease WRN) is a ubiquitously expressed nuclear protein belonging to the RecQ family. It functions as a helicase and exonuclease enzyme, and plays a crucial role in the formation of DNA replication centers. This protein is also involved in DNA repair and recombination, and may be involved in transcriptional events mediated by RNA polymerase II. It is regulated by phosphorylation and is targeted by the DNA-dependent protein kinase enzyme. Disrupted expression of the gene encoding the Werner’s syndrome helicase protein often results in the production of truncated Werner’s syndrome helicase proteins. Over 50 gene mutations may contribute to this effect—and lead to the development of Werner syndrome, a condition characterized by accelerated aging and early onset of age-related conditions such as cancer, atherosclerosis, and osteoporosis.

References

Product Information
Format	Purified
Control	HEK293 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.
Key Applications	Western Blotting

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human WRN.
Clone	20A11.1
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000544
Entrez Gene Summary	This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
Gene Symbol	WRN RECQ3 RECQL2
Purification Method	Protein G Purified
UniProt Number	Q14191
UniProt Summary	FUNCTION: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. COFACTOR: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro). SUBUNIT STRUCTURE: Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. SUBCELLULAR LOCATION: Nucleus › nucleolus. Nucleus. POST-TRANSLATIONAL MODIFICATION: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE: Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins. SEQUENCE SIMILARITIES: Belongs to the helicase family. RecQ subfamily. Contains 1 3'-5' exonuclease domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HRDC domain.
Molecular Weight	~162 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: A 1:2,000 dilution of this antibody detected Werner's syndrome helicase (WRN) in 10 µg of HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABD34	04053252594922

Documentation

Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1 SDS

Title
Safety Data Sheet (SDS)

Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1 Certificates of Analysis

Title	Lot Number
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2460445	2460445
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2467138	2467138
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2342886	2342886
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3172611	3172611
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3299950	3299950
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3393427	3393427
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3589804	3589804
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3808794	3808794
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3934339	3934339
Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 4206978	4206978

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New Products: Volume 3, 2012

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