Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

662099 Anti-Ubiquitin Rabbit pAb

This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin.

Anti-Ubiquitin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
662099
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Dr, E. coli, Fg, Gp, Ht, H, Mk, M, Rb, R, Sh, YeastRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
662099-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
Catalogue Number662099
Brand Family Calbiochem®
Application Data
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
References
ReferencesJungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.
Product Information
FormLiquid
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:1000, chemiluminescence)
Immunohistochemistry (1:200)
Immunoprecipitation (1:100)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogenbovine erytherocyte ubiquitin conjugated to KLH
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Chicken
  • Drosophila
  • E. coli
  • Frog
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
  • Sheep
  • Yeast
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
662099-50UL 04055977261189

Documentation

Anti-Ubiquitin Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Ubiquitin Rabbit pAb Certificates of Analysis

TitleLot Number
662099

References

Reference overview
Jungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2010 RFH
ApplicationImmunoblotting (1:1000, chemiluminescence)
Immunohistochemistry (1:200)
Immunoprecipitation (1:100)
Application Data
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
BackgroundUbiquitin is a highly conserved protein that functions in intracellular regulation. The ubiquitin system is the major pathway for selective protein degradation and is responsible for the degradation of abnormal and short-lived proteins. In the first step, ubiquitin is activated in an ATP-dependent reaction by ubiquitin-activating enzyme (E1). Activated ubiquitin is transferred to a ubiquitin conjugating enzyme (E2), then transferred to a protein substrate with the aid of ubiquitin-protein ligase (E3). An isopeptide linkage is formed between the C-terminus of the ubiquitin and an internal lysine residue of the substrate protein. Degradation requires the presence of a multi-ubiquitin chain on a substrate, which targets the protein for degradation by the 26S proteasome. Ubiquitin molecules are then released and recycled.
HostRabbit
Immunogen speciesBovine
Immunogenbovine erytherocyte ubiquitin conjugated to KLH
IsotypeIgG
SpeciesDrosophila, E. coli, bovine, canine, chicken, frog, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep, yeast
FormLiquid
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Toxicity Standard Handling
ReferencesJungmann, J., et al. 1993. Nature 361, 369.
Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.