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ABC499 Anti-Tropomyosin Antibody, isoforms 5a, 5b, Br2, Br3

This Anti-Tropomyosin Antibody, isoforms 5a, 5b, Br2, Br3 is validated for use in western blotting, IHC & ICC for the detection of Tropomyosin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Cytoskeleton Gene Symbol: Tpm1, Tpm-1, Tpma UniProt Number: P58771
ABC499
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IHC, ICCShAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC499
DescriptionAnti-Tropomyosin Antibody, isoforms 5a, 5b, Br2, Br3
Alternate Names
  • Tropomyosin alpha-1 chain
  • Alpha-tropomyosin
  • Tropomyosin-1
Background InformationTropomyosin, also known as Tropomyosin alpha-1 chain or Alpha-tropomyosin, or Tropomyosin-1, and encoded by the gene Tpm1/Tpm-1/TPMA, is one chain of the heterodimer complex of tropomyosin that binds to actin in muscle and non-muscle cells and plays a central role in the troponin complex in muscle regulating the calcium dependent vertebrate striated muscle contraction. In non muscle cells tropomyosin assists in cell structure by stabilizing the actin cytoskeleton filaments. Tropomyosin is localized to the cytoplasm. Tropomyosin is expressed in cardiac and skeletal muscle cells as well as non-muscle cells. Defects in Tropomyosin may be associated with Cardiomyopathy, familial hypertrophic 3 (CMH3)] disease. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter-and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
References
Product Information
FormatAffinity Purified
PresentationPurified sheep polyclonal in pH 7.0 buffer containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Tropomyosin Antibody, isoforms 5a, 5b, Br2, Br3 is validated for use in western blotting, IHC & ICC for the detection of Tropomyosin.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Tropomyosin in 10 µg of rat brain, human brain, mouse cerebellum, and mouse hypothalamus tissue lysate.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Tropomyosin in human prostate cancer tissue and human breast cancer tissue.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Tropomyosin in B35 cells (Schevzov, G., et al. (2011). Bioarchitecture. 1(4):135-164.).
Biological Information
ImmunogenLinear peptide corresponding to exon 1b of mouse Tropomyosin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostSheep
SpecificityThis antibody specifically recognizes isoforms 5a, 5b, Br2, and Br3 of Tropomyosin.
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tpm1
  • Tpm-1
  • Tpma
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~32 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC499 04053252944826