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AP1181
Sigma-AldrichAnti-TLR7 Mouse mAb (2C9)
This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7.
More>>This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7. Less<<
Anti-TLR7 Mouse mAb (2C9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
Clone
2C9
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-October-2010 RFH
Synonyms
Anti-Toll-like Receptor 7
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human TLR7 by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-TLR7 Mouse mAb (2C9) (Cat. No. AP1181) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~120 kDa TLR7 protein.
Background
Stimulation of the NFκB signaling pathway by TLR7 suggests that this protein plays a role in immune response.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
Clone
2C9
Isotype
IgG2b
Species
human
Positive control
IMR32 cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
