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04-889 Sigma-AldrichAnti-Src Antibody, clone N6L, rabbit monoclonal

This Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ASV, EC 2.7.10.2, OTTHUMP00000174476, OTTHUMP00000174477, SRC1, c-SRC, c-Src, c-src, p60-Src , pp60c-src UniProt Number: P12931

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	04-889
Replaces	05-889
Description	Anti-Src Antibody, clone N6L, rabbit monoclonal
Alternate Names	proto-oncogene tyrosine-protein kinase SRC protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src tyrosine-protein kinase SRC-1 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Background Information	pp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype

Product Information
Format	Culture Supernatant
Control	Included positive antigen control of non-stimulated A431 lysate (Cat. No. 12-301). Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation	Cultured supernantant containing 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src.
Key Applications	Western Blotting

Biological Information
Immunogen	N-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.
Clone	N6L
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn.
Isotype	IgG
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_005408.1
Entrez Gene Summary	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol	ASV EC 2.7.10.2 OTTHUMP00000174476 OTTHUMP00000174477 SRC1 c-SRC c-Src c-src p60-Src pp60c-src
UniProt Number	P12931
UniProt Summary	CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 By similarity. Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. Interacts with CAV2 (tyrosine phosphorylated form). PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Molecular Weight	~ 60 kDa

Product Usage Statements
Quality Assurance	Western Blot Analysis: A 1:500 to 1:1000 dilution of this antibody detected Src in RIPA lysates from A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µl

Global Trade Item Number
Catalogue Number	GTIN
04-889	04053252623479

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