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04-1014 Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: APRF, FLJ20882, HIES, MGC16063 UniProt Number: P40763
04-1014
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IH(P), IPRbUnpurifiedMonoclonal Antibody
Description
Catalogue Number04-1014
DescriptionAnti-STAT3 Antibody, clone E121-21, rabbit monoclonal
Alternate Names
  • Acute-phase response factor
  • DNA-binding protein APRF
  • signal transducer and activator of transcription 3
  • signal transducer and activator of transcription 3
    (acute-phase response factor)
Background InformationSTAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
References
Product Information
FormatUnpurified
HS Code3002 15 90
Control
  • A431 cell lysate
PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a previous lot detected STAT3 in lung squamous carcinoma tissue.
Immunocytochemistry Analysis: A 1:100 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:20 dilution from a previous lot was used in IP.
Biological Information
ImmunogenSynthetic peptide corresponding to residues surrounding Ser727 of human STAT3.
EpitopeSer727
CloneE121-21
HostRabbit
SpecificityThe antibody only detects STAT3 without phosphorylation on Ser727. It does not detect Ser727-phosphorylated STAT3.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to cross-react with bovine, and horse, based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APRF
  • FLJ20882
  • HIES
  • MGC16063
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. Interacts with SIPAR. Interacts (via SH2 domain) with NLK By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import By similarity. Interacts with TMF1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation.
TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.
INVOLVEMENT IN DISEASE: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.
SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Molecular Weight~92 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on A431 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect STAT3 in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1014 04053252729881

Documentation

Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-STAT3, clone E121-21, Rabbit Monoclonal - 2377648 2377648
Anti-STAT3, clone E121-21, Rabbit Monoclonal - 2161140 2161140
Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1628716 NG1628716
Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1675978 NG1675978
Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1726968 NG1726968
Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1823954 NG1823954

Brochure

Title
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

Technical Info

Title
JAK/STAT Signaling Research Focus