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09-038-I-25UG Anti-SOX17

Anti-SOX17, Cat. No. 09-038-I-25UG, is a highly specific rabbit polyclonal antibody, that targets Transcription factor SOX-17 and has been tested in Western Blotting and Immunofluorescence and Immunohistochemistry (Paraffin).

Anti-SOX17 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Sox-17 UniProt Number: Q61473
09-038-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MWB, IF, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number09-038-I-25UG
DescriptionAnti-SOX17
Alternate Names
  • Transcription factor SOX-17
Background InformationTranscription factor SOX-17 (UniProt: Q61473; also known as SOX-17) is encoded by the Sox-17 gene (Gene ID: 20671) in murine species. Sox17 protein is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5'-AACAAT-3 or 5'-AACAAAG-3' and modulates transcriptional regulation via WNT3A. Sox17 is reported to act upstream of the Notch system and downstream of the canonical Wnt system and acts as a link between the canonical Wnt and Notch signaling systems. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues like pancreatic and liver cells. SOX17 is the key regulator of human primordial germ cells (hPGSc) fate and is considered as one of the earliest markers. It is also shown to be the transcription factor for the regulation of oligodendrocyte progenitor cells, maintenance of fetal hematopoeitic stem cells, and in the mediation of cardiac muscle cell formation. SOX17 is selectively expressed in arterial endothelial cells at the early stages of embryonic development and maintains the same distribution postnatally and in the adult. Inactivation of SOX17 in endothelial cells in mouse embryo leads to a lack of arterial differentiation and vascular remodeling, which that results in embryo death in utero. (Ref.: Corada, M., et al. (2013). Nat. Communications 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SOX17, Cat. No. 09-038-I-25UG, is a highly specific rabbit polyclonal antibody, that targets Transcription factor SOX-17 and has been tested in Western Blotting and Immunofluorescence and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected SOX17 in 10 µg of human spinal cord tissue lysate.
Immunofluorescence Analysis: A 1:150-300 dilution from a representative lot detected  SOX17 in human testis and rat colon tissues.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected  SOX17 in human prostate, mouse stomach, and rat uterus tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of murine Sox17.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Sox17 in human, mouse, and rat. It targets an epitope within 17 amino acids from the N-terminal region.
Species Reactivity
  • Rat
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat
Antibody TypePolyclonal Antibody
Gene Symbol
  • Sox-17
Purification MethodAffinity Purified
UniProt Number
Molecular Weight40 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in H9 human stem cells.
Western Blotting Analysis: 2 µg/mL of this antibody detected SOX17 in 10 µg of H9 human stem cells lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
09-038-I-25UG 04054839441585