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05-822 Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

05-822
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWBRbPurifiedMonoclonal Antibody
Description
Catalogue Number05-822
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
Product Information
FormatPurified
Quality LevelMQ100
Applications
ApplicationAnti-Rho (-A Antibody, -B, -C), clone 3L74 is an antibody against Rho (-A, -B, -C) for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
Cloneclone 3L74
HostRabbit
SpecificityRecognizes Rho (-A, -B, -C)
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteBroad species cross-reactivity expected based on sequence homology
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RHOA
  • RHO12
  • Rho12
  • RhoA
  • RHOH12
  • H12
  • ARHA
  • ARH12
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1.
COFACTOR: Magnesium.
SIZE: 193 amino acids; 21768 Da
SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
DOMAIN: SwissProt: P61586 The basic-rich region is essential for yopT recognition and cleavage.
PTM: Substrate for botulinum ADP-ribosyltransferase. & Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
Molecular WeightMr 22kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20°C from date of shipment
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
05-822 04053252403170