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MABN77 Anti-Potassium Channel Kv1.2 Antibody, clone K14/16

MABN77
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHC, WB, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN77
DescriptionAnti-Potassium Channel Kv1.2 Antibody, clone K14/16
Alternate Names
  • potassium voltage-gated channel, shaker-related subfamily, member 2
  • potassium voltage-gated channel subfamily A member 2
  • Voltage-gated potassium channel subunit Kv1.2
  • Voltage-gated potassium channel HBK5
  • potassium channel
  • Voltage-gated K(+) channel HuKIV
  • voltage-gated potassium channel protein Kv1.2
Background InformationPotassium channel Kv1.2 is one of many different types of potassium channels in the cell. The Kv family of potassium channels seem to have a conserved homotetramer formation consisting of four voltage sensors and one pore domain. Kv1.2 cooperates with cortactin (an actin cytoskeleton-binding protein) and together they may have a part in the regulation of the ionic current.
Product Information
FormatPurified
Control
  • Rat brain membrane tissue lysate
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Potassium Channel Kv1.2 Antibody, clone K14/16 is validated for use in IH, WB, IF for the detection of Potassium Channel Kv1.2.
Key Applications
  • Immunohistochemistry
  • Western Blotting
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: a previous lot of this antibody was used by an independent laboratory in IF. (Yang, J.W., et al. (2007). PNAS. 104(50):20055–20060.)
Biological Information
ImmunogenRecombinant protein corresponding to rat Kv1.2.
CloneK14/16
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryDelayed-rectifier K+ channel expressed in heart and brain [RGD].
Gene Symbol
  • KCNA2
  • HBK5
  • RBK2
  • HK4
  • MK2
  • KV1.2
  • NGK1
  • HUKIV
  • Kv1.2
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

SUBUNIT STRUCTURE: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4.

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

DOMAIN: The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

SEQUENCE SIMILARITIES: Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily. [View classification]
Molecular Weight~ 65 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kv1.2 on 10 µg of rat brain membrane tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MABN77 04053252422553