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MABT1496-25UG Anti-Plakophilin-3 Antibody, clone 7F6

Anti-Plakophilin-3, clone 7F6, Cat. No. MABT1496, is a mouse monoclonal antibody that detect Plakophilin-3 and has been tested for use in Western Blotting.

Anti-Plakophilin-3 Antibody, clone 7F6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: PKP3 UniProt Number: Q9Y446
MABT1496-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1496-25UG
DescriptionAnti-Plakophilin-3 Antibody, clone 7F6
Alternate Names
  • PKP3
  • Plakophilin 3
Background InformationPlakophilin-3 (UniProt: Q9Y446; also known as PKP3) is encoded by the PKP3 gene (Gene ID: 11187) in human. Plakophilin 3 a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells and dendritic reticular cells of lymphatic follicles. Plakophilin-3 is an Armadillo-like protein whose expression pattern is largely restricted to epithelial cell types and plays a role in junctional plaques. It is localized in the desmosomal plaques and in the nucleus. Plakophilin-3 contains a central Arm repeat domain, containing 10 Arm repeats and a short C-terminal domain. Plakophilin proteins act in a cellular desmosome-dependent adhesion and signaling pathways. It is shown to bind directly to the desmosomal cadherins Dsg1, Dsg2, Dsg3, Dsc3a, and Dsc3b, and possibly also to Dsc1a and Dsc2a. Two isoforms of Plakophiln-3 have been described that are produced by alternative splicing. Isoform PKP3a is found in desmosomes of most simple and stratified epithelia and PKP3b is abundant in the stratified epithelial cells, but not in simple epithelial cells. Plakophilin-3 levels are up-regulated in lung carcinogenesis. (Ref.: Bonne, S., et al. (1999). J. Cell Sci. 112(1); 2265-2276; Furukawa, C., et al. (2005). Cancer Res. 65(16); 7102-7110).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Plakophilin-3, clone 7F6, Cat. No. MABT1496, is a mouse monoclonal antibody that detect Plakophilin-3 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenMBP-tagged recombinant fragment corresponding to the first 308 amino acids from human Plakophilin-3.
Clone7F6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7F6 specifically detects human Plakophilin-3. It targets an epitope with in the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PKP3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~95 kDa observed; 87.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin-3 in HaCaT cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1496-25UG 04054839557927