Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE1151 Anti-OASIS (CREB3L1) Antibody, clone 10H1

Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-OASIS (CREB3L1) Antibody, clone 10H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CREB3L1, OASIS, PSEC0238 UniProt Number: Q96BA8
MABE1151
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1151
DescriptionAnti-OASIS (CREB3L1) Antibody, clone 10H1
Alternate Names
  • Cyclic AMP-responsive element-binding protein 3-like protein 1
  • cAMP-responsive element-binding protein 3-like protein 1
  • Old astrocyte specifically-induced substance
  • OASIS
Background InformationCyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in
unfolded protein response (UPR). It binds the DNA consensus sequence 5'-GTGXGCXGC-3'. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues.

Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).

Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).
Biological Information
ImmunogenKLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1).
Clone10H1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat, Mouse, Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CREB3L1
  • OASIS
  • PSEC0238
Purification MethodProtein G purified
UniProt Number
Molecular Weight57.00 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human prostate cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1151 04054839396670