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MABF2097-100UG Anti-Norovirus (MNV-1) Antibody, clone 5C4.10

Anti-Norovirus (MNV-1), clone 5C4.10, Cat. No. MABF2097, is a mouse monoclonal antibody that detects murine Norovirus capsid protein and has been tested for use in ELISA and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
MABF2097-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VirusELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2097-100UG
DescriptionAnti-Norovirus (MNV-1) Antibody, clone 5C4.10
Alternate Names
  • MNV-1
  • Murine Norovirus
Background InformationNorovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187 kDa polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59 kDa capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22 kDa basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. This monoclonal antibody, clone 5C4.10, recognizes the S domain portion of the viral capsid protein and is broadly reactive with pan-noroviruses of murine and human type. (Ref.: Kolawole AO, et al. (2017). mSphere 2: e00334-17; Kolawole, AO et al. (2014). J. Gen. Virol. 95(9); , 1958 1968; Royall, E., et al. (2015). J. Biol. Chem. 290(8); 4748-58; Wobus, CE et al. (2006). J. Virol. 80(11); 5104-5112).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Norovirus (MNV-1), clone 5C4.10, Cat. No. MABF2097, is a mouse monoclonal antibody that detects murine Norovirus capsid protein and has been tested for use in ELISA and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Norovirus (MNV-1) in Western Blotting applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

ELISA Analysis: A representative lot detected Norovirus (MNV-1) in ELISA applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68; Kolawole, A.O., et. al. (2017). mSphere. 2(5). pii: e00334-17).
Biological Information
ImmunogenInfectious and UV-inactivated murine norovirus-1.
Clone5C4.10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5C4.10 specifically recognizes the S domain portion of capsid protein of murine Norovisrus.
IsotypeIgG2bκ
Species Reactivity
  • Virus
Species Reactivity NoteNorovirus.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Molecular Weight~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in RAW 264.7 cells infected with Murine norovirus-1.CW3.

Western Blotting Analysis: 1 µg/mL of this antibody detected Norovirus (MNV-1) in RAW 264.7 cells infected with Murine norovirus-1.CW3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2097-100UG 04054839540240

Documentation

Anti-Norovirus (MNV-1) Antibody, clone 5C4.10 Certificates of Analysis

TitleLot Number
Anti-Norovirus (MNV-1), clone 5C4.10 - 3291374 3291374
Anti-Norovirus (MNV-1), clone 5C4.10 - 3609951 3609951
Anti-Norovirus (MNV-1), clone 5C4.10 - 3751854 3751854
Anti-Norovirus (MNV-1), clone 5C4.10 - 3938565 3938565
Anti-Norovirus (MNV-1), clone 5C4.10 - 4045772 4045772
Anti-Norovirus (MNV-1), clone 5C4.10 - 4118022 4118022
Anti-Norovirus (MNV-1), clone 5C4.10 - 4243334 4243334
Anti-Norovirus (MNV-1), clone 5C4.10 Monoclonal Antibody Q3084578