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ABN1459 Anti-Nkx6.1

Anti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Nkx6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NKX6-1, NKX6A UniProt Number: P78426
ABN1459
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1459
DescriptionAnti-Nkx6.1
Alternate Names
  • Homeobox protein Nkx-6.1
  • Homeobox protein NK-6 homolog A
Background InformationHomeobox protein Nkx-6.1 (UniProt: P78426; also known as Homeobox protein NK-6 homolog A) is encoded by the NKX6-1 (also known as NKX6A) gene (gene ID: 4825) in human. Nkx-6.1 is a transcription factor that binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. It is expressed in pancreatic beta cells and is involved in transcriptional regulation in islet beta cells. During pancreatic development both Pdx-1 and Nkx-6.1 are observed in rat pancreas. However, later Nkx-6.1 became more beta-cell-restricted, and Pdx-1 is found in other islet cell types and in the duodenal epithelium. Nkx-6.1 binds to the insulin promoter and is involved in regulation of the insulin gene. The C-terminal domain of Nkx-6.1 contributes to sequence-specific DNA-binding. Together with NKX2-2 and IRX3 it acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by Sonic hedgehog (Shh) signals. Although Shh signaling is required for Nkx-6.1 expression in the ventral neural tube and spinal meninges, a Shh-independent pathway appears to operate in regulating Nkx-6.1 expression in the foregut region. (Ref.: Cai, J., et al. (2000). Genesis. 27(1); 6-11; Oster, A., et al. (1998). J. Histochem. Cytochem. 46(6); 707-715).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Nkx6.1 in human pancreas and mouse pancreas tissue sections.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 61 amino acids from the N-terminal region of human Homeobox protein Nkx-6.1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Homeobox protein Nkx-6.1 in human cells. It targets an epitope within 61 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NKX6-1
  • NKX6A
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~54 kDa observed; 37.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Nkx6.1 in PANC-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1459 04054839415081