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05-724 Anti-Myc Tag Antibody, clone 4A6

05-724
200 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IF, IP, WB, ChIP-seqMPurifiedMonoclonal Antibody
Description
Catalogue Number05-724
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Myc Tag Antibody, clone 4A6
Background InformationIdentification of the target epitope is important for characterization of a monoclonal antibodies. Epitope tags are short, linear antibody recognition sequences that enable detection of tagged fusion proteins by antibodies. Over the years, a wide range of anti-tag antibodies have been developed and each one of these offer unique advantages and disadvantages. Epitope tags can undergo post-translational modifications, such as phosphorylation and glycosylation in cells and cause an increase in the molecular mass of the fused protein, which can result in changes in gel mobility. Epitope tags of smaller number of amino acid residues are advantageous in that they exhibit minimal side effects on structure and function of fused target protein. A Myc tag can be used in many different immunoassays and is useful when there is no antibody against the protein of interest. Adding a Myc-tag permits the detection of such proteins with an antibody against the Myc epitope. Clone 4A6 is an antibody that recognizes ten amino acid sequence EQKLISEEDL (Glu-Gln-Lys-Leu-Iso-Ser-Glu-Glu-Asp-Leu) corresponding to the C-terminal region of human c-Myc protein (UniProt: P01106). Unlike other clones whose performance may be affected when Myc tag sequence is followed by smaller neutral amino acids, Clone 4A6 offers much more consistent detection of Myc-tagged proteins irrespective of any changes in neighboring amino acid sequences. (Ref. Schuchner, S., et al. (2020). Sci. Signal. 13(616); eaax9730; Charest-Morin, X., et al. (2013). Front. Pharmacol. 4: 122).
Product Information
FormatPurified
Control
  • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
PresentationProtein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Quality LevelMQ100
Applications
ApplicationAnti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP, and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
  • ChIP-seq
Biological Information
Immunogenpeptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Clone4A6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
Gene Symbol
  • MYC
  • C-MYC
  • c-Myc
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Molecular WeightPredicted MW for human Myc: 49 kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2 to 8°C for 1 year from date of shipment.
Packaging Information
Material Size200 µg
Global Trade Item Number
Catalogue Number GTIN
05-724 08436037125744