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MAB3319 Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6

MAB3319
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3319
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-14 Antibody, catalytic domain, clone 114-6G6
Alternate Names
  • MT1-MMP
Product Information
FormatPurified
PresentationLiquid in 0.1 M sodium phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin.
Quality LevelMQ100
Applications
ApplicationThis Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6 is validated for use in ELISA, WB, IH(P) for the detection of MMP-14.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunoblotting: 8-20 μg/mL. 66+60kDa (Ueno et al., 1997)

Immunohistochemistry on paraffin-embedded tissue sections, use at 20 μg/mL concentration (see Ueno et al., 1997; Nakamura et al., 1999). Requires periodate-lysine-paraformaldehyde fixative (Namura et al., 1999) for 18-24 hrs. at 4°C.

EIA Optimal working dilutions must be determined by end user.
Biological Information
Epitopecatalytic domain
Clone114-6G6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody specifically reacts with human MT1-MMP. This is a purified mouse monoclonal antibody to an oligopeptide of REVPYAYIREGHEK (residue 160-173) on human membrane-type 1 matrix metalloproteinase (human MT1-MMP) and does not react with mouse or rat. However, this sequence is identical in mouse and rat MT1-MMP. For unknown reasons this antibody does not react on westerns to CC1043.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Gene Symbol
  • MMP14
  • MTMMP1
  • MMP-14
  • MT1MMP
  • MT1-MMP
  • MMP-X1
  • EC 3.4.24.80
Non-Reactive Species
  • Mouse
  • Pig
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • Manufactured by Daiichi Fine Chemical Co., Ltd
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB3319 04053252612077