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ABS44 Anti-MAPK 1/2 Antibody

Detect MAPK 3/MAPK 1 (ERK1/ERK2) using this Anti-MAPK 1/2 Antibody validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-MAPK 1/2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: Erk1, Erk2, Mapk, Mapk1, Mapk3, Prkm1, Prkm3 UniProt Number: P21708
ABS44
100 µg  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, HWB, IP, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS44
Replaces06-182
DescriptionAnti-MAPK 1/2 Antibody
Alternate Names
  • ERK-1
  • ERT2
  • Extracellular signal-regulated kinase 1
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 3
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 3
  • MNK1
  • p44-ERK1
  • p44-MAPK
  • ERK-2
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAP kinase isoform p42
  • MAPK 1
  • MAPK 2
  • Mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p42-MAPK
Background InformationMitogen-activated protein kinase 3 (EC 2.7.11.24; UniProt P21708; also known as ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, MNK1, p44-ERK1, p44-MAPK) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID 50689) in rat species. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and β-Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • A431 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MAPK 3/MAPK 1 (ERK1/ERK2) using this Anti-MAPK 1/2 Antibody validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in BALB/3T3, A431, and L6 cell lysates.



Western Blotting (SNAP ID) Analysis: 1-2 µg/mL from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in BALB/3T3, A431, and L6 cell lysates.



Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in NIH/3T3, A431, HeLa, and C2C12 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal sequence of rat MAPK 3 (ERK-1).
EpitopeC-terminal
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes MAPK 3/MAPK 1 (ERK1/ERK2) C-terminal sequence.
Species Reactivity
  • Rat
  • Mouse
  • Human
Species Reactivity NoteReacts with human, mouse, and rat. Expected to react with bovine and canine based on 94% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Erk1
  • Erk2
  • Mapk
  • Mapk1
  • Mapk3
  • Prkm1
  • Prkm3
Purification MethodProtein A purfied.
UniProt Number
Molecular Weight~44/42 kDa observed. 41.26/36.30 kDa (human MAPK1 isoform 1/2), 41.14 kDa (rat MAPK1), 41.14 kDa (mouse MAPK1), 43.00/38.14 kDa (human MAPK3 isoform 1/2), 42.95/45.64 kDa (rat MAPK3 isoform 1/2), 42.93 kDa (mouse MAPK3) calculated (Met1 removed). Target sequence is not present in human MAPK3 spliced isoform 3 (ERK1b).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.



Western Blotting Analysis: 0.5 µg/mL of this antibody detected MAPK 3/MAPK 1 (ERK1/ERK2) in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS44 04053252324109

Documentation

Anti-MAPK 1/2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-MAPK 1/2 Antibody Certificates of Analysis

TitleLot Number
Anti-MAPK 1/2 - 2420429 2420429
Anti-MAPK 1/2 - 2052352 2052352
Anti-MAPK 1/2 - 3170189 3170189
Anti-MAPK 1/2 - 3246190 3246190
Anti-MAPK 1/2 - 3273613 3273613
Anti-MAPK 1/2 - 3321123 3321123
Anti-MAPK 1/2 - 3422077 3422077
Anti-MAPK 1/2 - 3746420 3746420
Anti-MAPK 1/2 - 3859712 3859712
Anti-MAPK 1/2 - 3959504 3959504
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Brochure

Title
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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