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MABS816 Anti-MAP3K2, clone 4B4 Antibody

MABS816
100 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS816
Replaces04-378
DescriptionAnti-MAP3K2, clone 4B4 Antibody
Alternate Names
  • Mitogen-activated protein kinase kinase kinase 2
  • MAP3K2
Background InformationMitogen-activated protein kinase kinase kinase 2 (MAP3K2) is a member of serine/threonine protein kinase family. MAP3K2 preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. MAP3K2 has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-MAP3K2, clone 4B4 Antibody is an antibody against MAP3K2 for use in Western Blotting, Immunocytochemistry, ELISA.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected MAP3K2 in 3T3-L1 cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Biological Information
ImmunogenPurified recombinant fragment of human ERK3 expressed in E. Coli. 
Clone4B4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MAP3K2
  • MAPKKK2
  • MEKK2
Purification MethodUnpurified
UniProt Number
Molecular Weight~58 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 and MAP3K2 -hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP3K2 in hIgGFc transfected HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS816 04055977172348