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CBL218 Anti-Keratin K3/K76 Antibody, clone AE5

CBL218
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RbWB, IHC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL218
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Keratin K3/K76 Antibody, clone AE5
Product Information
FormatPurified
PresentationThe monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRabbit corneal epithelial keratin
CloneAE5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rabbit
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Gene Symbol
  • KRT3
  • CK3
  • K3
  • CK-3
  • Cytokeratin-3
  • Keratin-3
Purification MethodIon-exchange chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q01546 # Probably contributes to terminal cornification.
SIZE: 638 amino acids; 65871 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins.
DEVELOPMENTAL STAGE: Synthesized during maturation of epidermal keratinocytes.
SIMILARITY: SwissProt: Q01546 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Molecular Weight64 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
CBL218 04053252361777