Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC1751-100UL Anti-KISS1 Antibody, clone 6A4.27

MABC1751-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1751-100UL
DescriptionAnti-KISS1 Antibody, clone 6A4.27
Alternate Names
  • Metastasis-suppressor KiSS-1
  • Kisspeptin-1
Background InformationMetastasis-suppressor KISS-1 (UniProt: Q15726; also known as Kisspeptin-1) is encoded by the KISS1 (also known as PP5098) gene (Gene ID: 3814) in human. KISS1 is highly expressed in placenta and some expression is also observed in testis, pancreas, liver, small intestine. Low expression levels are also observed in the brain. Its expression is shown to increase in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Its expression is reported to be higher levels in first trimester trophoblasts than at term of gestation. KISS-1 is a secreted metastasis suppressor that demonstrates inhibition of metastases formation in several types of cancers. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form. Following its release, it is processed into several of peptides known as kisspeptins. It also generates a C-terminally amidated peptide known as metastin that acts as an endogenous ligand of GPR54, a GPCR. Activation of this receptor inhibits cell proliferation and cell migration that contributes to its tumor suppressor role. KISS1 expression is reported to be significantly reduced in human pancreatic cancer tissue and this reduced expression is associated with advanced disease. KISS1 also diminishes MMP9 expression by effecting reduced NF-kappa-B binding to the promoter. (Ref.: McNally, LR., et al. (2010). Clin. Exp. Metastasis. 27(8); 591-600).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-KISS1, clone 6A4.27, Cat. No MABC1751, is a mouse monoclonal antibody that detects Metastasis-suppressor KiSS-1 and is tested for use in Immunohistochemistry (Paraffin) and Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KISS1 in human pancreatic cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected KISS1 in Immunohistochemistry applications (McNally, L.R., et. al. (2010). Clin Exp Metastasis. 27(8):591-600; Ulasov, I.V., et. al. (2012). Cancer. 118(8):2096-105).

Western Blotting Analysis: A representative lot detected KISS1 in Western Blotting applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).

Immunoprecipitation Analysis: A representative immunoprecipitated KISS1 in Immunoprecipitation applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).
Biological Information
ImmunogenA linear peptide corresponding to 54 amino acids from the C-terminal half of human Kisspeptin-1 (KISS1).
Clone6A4.27
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6A4.27 is a mouse monoclonal antibody that detects Kisspeptin-1. It targets an epitope with in the KP54 (G68-F121) region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KISS1
  • PP5098
Purification MethodProtein G purified
UniProt Number
Molecular Weight14.70 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KISS1 in human placenta tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1751-100UL 04054839986789

Documentation

Anti-KISS1 Antibody, clone 6A4.27 SDS

Title

Safety Data Sheet (SDS) 

Anti-KISS1 Antibody, clone 6A4.27 Certificates of Analysis

TitleLot Number
Anti-KISS1, clone 6A4.27 - Q3222080 Q3222080

References

Reference overviewPub Med ID
Furin is the major proprotein convertase required for KISS1-to-Kisspeptin processing.
Harihar, S; Pounds, KM; Iwakuma, T; Seidah, NG; Welch, DR
PLoS One  9  e84958  2014

Show Abstract
24454770 24454770
Clinical significance of KISS1 protein expression for brain invasion and metastasis.
Ulasov, IV; Kaverina, NV; Pytel, P; Thaci, B; Liu, F; Hurst, DR; Welch, DR; Sattar, HA; Olopade, OI; Baryshnikov, AY; Kadagidze, ZG; Lesniak, MS
Cancer  118  2096-105  2012

Show Abstract
21928364 21928364
KISS1 over-expression suppresses metastasis of pancreatic adenocarcinoma in a xenograft mouse model.
McNally, LR; Welch, DR; Beck, BH; Stafford, LJ; Long, JW; Sellers, JC; Huang, ZQ; Grizzle, WE; Stockard, CR; Nash, KT; Buchsbaum, DJ
Clin Exp Metastasis  27  591-600  2010

Show Abstract
20844932 20844932