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MAB413-I-100UG Anti-Interferon-α Antibody, clone ST126

Anti-Interferon-a, clone ST126, Cat. No. MAB413-I, is a mouse monoclonal antibody that detects Interferon alpha-2 and is tested for use in ELISA, Immunohistochemistry (Paraffin), Neutralizing, Radioimmunoassay, and Western Blotting.

MAB413-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB413-I-100UG
DescriptionAnti-Interferon-α Antibody, clone ST126
Alternate Names
  • Interferon alpha-2
  • IFN-alpha-2
  • Interferon alpha-A
  • LeIF A
Background InformationInterferon alpha-2 (UniProt: P01563; also known as IFN-alpha-2, Interferon alpha-A, LeIF A) is encoded by the IFNA2 (also known as IFNA2A, IFNA2B, IFNA2C) gene (Gene ID: 3440) in human. Interferons are divided into three families (type I, II, and III) based on their sequence homology. Type I is the largest group that includes Interferons a and b are the primary defensive cytokines against pathogens and display strong antiviral activities. They also display anti-bacterial and anti-cancer properties. IFN-a is rapidly induced and secreted by cells to limit virus replication through numerous and varied mechanisms, including the induction of intrinsic restriction factors. IFN-a2 is produced by macrophages with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature active form. It is produced in response to viral infection and triggers the induction of protective genes that inhibit viral replication and impede viral dissemination. IFN-a2 is shown to be an effective agent against hepatitis viruses. Chronic administration of IFN-a is shown to cause the regression of vascular neoplasms, including hemangiomas, Kaposi's sarcoma, and pulmonary hemangiomatosis. (Ref.: Lavender, KJ., et al. (2016). J, Virol. 90(13); 6001-6013).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Interferon-a, clone ST126, Cat. No. MAB413-I, is a mouse monoclonal antibody that detects Interferon alpha-2 and is tested for use in ELISA, Immunohistochemistry (Paraffin), Neutralizing, Radioimmunoassay, and Western Blotting.
Key Applications
  • Western Blotting
  • Neutralizing
  • Radioimmunoassay
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesNeutralizing: A representative lot of this antibody neutralized the antiviral activity of Interferon-a. (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

ELISA Analysis: A representative lot detected Interferon-a in ELISA applications (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

Radioimmunoassay: A representative lot detected Interferon-a in Radioimmunoassay applications (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Interferon-a in human kidney and human stomach cancer tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA full-length purified human interferon-a produced by human Burkitt lymphoma Namalwa cell line.
EpitopeUnknown
CloneST126
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone ST126 is a mouse monoclonal antibody that detects human Interferon a.
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IFNA2
  • IFNA2A
  • IFNA2B
  • IFNA2C
Purification MethodProtein G purified
UniProt Number
Molecular Weight~19 kDa observed; 21.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human IFN- a2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human IFN-a2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB413-I-100UG 04065265944823