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MAC140 Anti-Inntag™ 10 (IT10) Antibody, clone IT10

Anti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.

Anti-Inntag™ 10 (IT10) Antibody, clone IT10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: N/A UniProt Number: P10414
MAC140
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC140
DescriptionAnti-Inntag™ 10 (IT10) Antibody, clone IT10
Alternate Names
  • Allergen Amb t V
  • Allergen Ra5G
  • Amb t 5
Background InformationPollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag IT10 is based on a packed beta-sheet conformation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Inntag 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunocytochemistry Analysis: A representative lot detected Inntag 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
ImmunogenHis-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.
CloneIT10-1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteAmbrosia trifida.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N/A
Purification MethodProtein G purified
UniProt Number
Molecular Weight~33.7 kDa observed; 8.06 kDa calculated. 33.7 kD observed corresponds to 29.4 kDa GFP plus 4.3 kDa IT10. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 10 (IT10) in 10 µg of transfected HEK293/GFP-IT10 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAC140 04054839240591