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05-784R Sigma-AldrichAnti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
Technical Information

Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: IRS1, IRS-1, HIRS-1 UniProt Number: P35570

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R, H	WB, IP	Rb	Purified	Monoclonal Antibody

Description
Catalogue Number	05-784R
Description	Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
Alternate Names	insulin receptor substrate 1
Background Information	IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.

References

Product Information
Format	Purified
Control	3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
Presentation	Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.
Quality Level	MQ100

Applications
Application	Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates. Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.

Biological Information
Immunogen	KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
Epitope	C-terminus
Clone	58-10C-31
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Antibody recognizes the C-terminus of IRS1.
Isotype	IgG
Species Reactivity	Mouse Rat Human
Species Reactivity Note	Reacts with mouse, rat and human.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_0371
Gene Symbol	IRS1 IRS-1 HIRS-1
Purification Method	Protein A Purfied
UniProt Number	P35570
UniProt Summary	FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3 SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats. Phosphorylation of Tyr-895 is required for GRB2-binding. SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain. Contains 1 PH domain.
Molecular Weight	~ 160 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates. Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
05-784R	04053252373909

Documentation

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal SDS

Title
Safety Data Sheet (SDS)

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal Certificates of Analysis

Title	Lot Number
Anti-IRS1, clone 58-10C-31	2475663
Anti-IRS1, clone 58-10C-31 - 2459055	2459055
Anti-IRS1, clone 58-10C-31 - 2009444	2009444
Anti-IRS1, clone 58-10C-31 - 2150090	2150090
Anti-IRS1, clone 58-10C-31 - 2207201	2207201
Anti-IRS1, clone 58-10C-31 - 2299140	2299140
Anti-IRS1, clone 58-10C-31 - 2302240	2302240
Anti-IRS1, clone 58-10C-31 - 2377415	2377415
Anti-IRS1, clone 58-10C-31 - 2563501	2563501
Anti-IRS1, clone 58-10C-31 - NG1861488	NG1861488

Brochure

Title
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

Technical Info

Title
Protein Blotting Handbook: 6th Edition

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