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MABC1106-25UG Anti-Hsp90 alpha Antibody, clone 1G6-D7

Anti-Hsp90 alpha, clone 1G6-D7, Cat. No. MABC1106, is a mouse monoclonal antibody that detects Hsp90 alpha, Hsp90 beta and has been tested for use in ELISA, Immunoprecipitation, Functional assay, Inhibition, and Western Blotting.

Anti-Hsp90 alpha Antibody, clone 1G6-D7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: HSP90AA1, HSP90A, HSPC1, HSPCA UniProt Number: P07900
MABC1106-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, IP, Function Assay, Inhibition, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1106-25UG
DescriptionAnti-Hsp90 alpha Antibody, clone 1G6-D7
Alternate Names
  • Heat shock protein HSP 90-alpha
  • Heat shock 86 kDa
  • HSP 86
  • Lipopolysaccharide-associated protein 2
  • LAP-2
  • LPS-associated protein 2
  • Renal carcinoma antigen NY-REN-38
Background InformationHeat shock protein HSP 90-alpha (UniProt: P07900; also known as Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38) is encoded by the HSP90AA1 (also known as HSP90A, HSPC1, HSPCA) gene (Gene ID: 3320) in human. HSP90 serves as a molecular chaperone that maintains the stability and functionality of numerous client proteins in an ATPase-dependent manner. It is known to interact dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle, and chaperone function. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. HSP90 and its co-chaperones are also shown to modulate transcription at least at three different levels (a) by altering the steady-state levels of certain transcription factors in response to various physiological cues (b) modulating the activity of certain epigenetic modifiers, such as histone deacetylases, and (c) by participation in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. In its resting state, through the dimerization of its C-terminal domain, HSP90 forms a homodimer, which is defined as the open conformation. Upon ATP-binding, the N-terminal domain undergoes significant conformational changes and comes in contact to form an active closed conformation. Following the completion of its chaperoning activity, ATP molecule is hydrolyzed to ADP which then dissociates from HSP90 and directs the protein back to the resting state. This monoclonal antibody (clone 1G6-D7) is reported to inhibit both de novo tumor formation and expansion of already formed tumors in mice. (Ref.: Li, W., et al. (2012). Biochim. Biophys. Acta. 1823(3); 730 741; Zou, M., et al. (2017). Oncogene 36(15); 2160-2171).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-Hsp90 alpha, clone 1G6-D7, Cat. No. MABC1106, is a mouse monoclonal antibody that detects Hsp90 alpha, Hsp90 beta and has been tested for use in ELISA, Immunoprecipitation, Functional assay, Inhibition, and Western Blotting.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Function Assay
  • Inhibition
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Hsp90 alpha in Immunoprecipitation applications (Dong, H., et. al. (2016). Sci Rep. 6:20605).

ELISA Analysis: A representative lot detected Hsp90 alpha in ELISA applications (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

Affects Function Analysis: A representative lot blocked parental MDA-MB-231 cell invasion in a dose-dependent manner. (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

Western Blotting Analysis: A representative lot detected Hsp90 alpha in Western Blotting applications (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

Inhibits Activity/Function Analysis: A representative lot inhibited both de novo tumor formation and expansion of already formed tumors in mice. (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).
Biological Information
ImmunogenA recombinant fragment corresponding to 115 amino acids from the F-5 region of human HSP90 alpha.
Clone1G6-D7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1G6-D7 is a mouse monoclonal antibody that detects HSP90 alpha and HSP90 beta in human cells. it targets an epitope within 9 amino acids from the internal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSP90AA1
  • HSP90A
  • HSPC1
  • HSPCA
Purification MethodProtein G purified
UniProt Number
Molecular Weight~90 kDa observed; 84.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Hsp90 alpha in MDA-MB-231 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1106-25UG 04054839531989