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MABN2512-25UG Anti-GABA(A) receptor subunit α-1 Antibody, clone 1F4

Anti-GABA(A) receptor subunit -1, clone 1F4, Cat. No. MABN2512, is a mouse monoclonal antibody that detects GABA(A) receptor subunit -1 and is tested for use in Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry, and Radioimmunoassay.

MABN2512-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2512-25UG
DescriptionAnti-GABA(A) receptor subunit α-1 Antibody, clone 1F4
Alternate Names
  • Gamma-aminobutyric acid receptor subunit alpha-1
  • GABA A receptor alpha-1
Background InformationGamma-aminobutyric acid receptor subunit 1 (UniProt: P14867; also known as GABA(A) receptor subunit alpha-1) is encoded by the GABRA1 gene (Gene ID: 2554) in human. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. On binding of two GABA molecules to the extracellular receptor domain, a conformational change is induced in the oligomer and Cl moves into the cell leading to an inhibitory hyperpolarization. GABAA and GABAB receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABA 1 receptor subunit is part of the heteropentamer formed by a combination of 1, β2, and 2 subunits. GABA receptor 1 subunit is synthesized with a signal peptide (aa 1-29) which is subsequently cleaved off to generate the mature form that contains four transmembrane domains (aa 252-273; 279-300; 313-334; and 422-443), one extracellular domain (aa 28-251), and a cytoplasmic domain (aa 335-421). (Ref.: Zhu, S., et al. (2018). Nature. 559(7712); 67-72; Goetz, T., et al. (2007). Prog. Brain Res. 160; 21-41).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-GABA(A) receptor subunit -1, clone 1F4, Cat. No. MABN2512, is a mouse monoclonal antibody that detects GABA(A) receptor subunit -1 and is tested for use in Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry, and Radioimmunoassay.
Key Applications
  • Immunocytochemistry
  • Radioimmunoassay
  • ELISA
  • Electron Microscopy
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected GABA(A) receptor subunit -1 in SH-SY5Y cells.

Radioimmunoassay: A representative lot detected GABA(A) receptor subunit -1 in Radioimmunoassay applications (Zhu, S., et. al. (2018). Nature. 559(7712):67-72).

ELISA Analysis: A representative lot detected GABA(A) receptor subunit -1 in ELISA applications (Zhu, S., et. al. (2018). Nature. 559(7712):67-72).

Electron Microscopy: A representative lot detected GABA(A) receptor subunit -1 in Electron Microscopy applications (Kim, J.J., et. al. (2020). Nature. 585(7824); 303-308).

).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHuman pentameric ( 1β2 2) GABA-A receptor protein.
EpitopeUnknown
Clone1F4
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1F4 is a mouse monoclonal antibody that detects GABA receptor -1.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GABRA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight51.80 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in SK-N-SH cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected GABA(A) receptor subunit -1, in SK-N-SH cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2512-25UG 04065265324274