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09-860 Anti-DDX3X Antibody

Use Anti-DDX3X Antibody (Rabbit Polyclonal Antibody) validated in WB to detect DDX3X also known as ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal.

Anti-DDX3X Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Binding Protein (RBP) Gene Symbol: CAP-Rf , DBX , DDX14 , DDX3, EC 3.6.1.- , HLP2 UniProt Number: O00571
09-860
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbSerumPolyclonal Antibody
Description
Catalogue Number09-860
DescriptionAnti-DDX3X Antibody
Alternate Names
  • ATP-dependent RNA helicase DDX3X
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
  • DEAD box protein 3, X-chromosomal
  • DEAD box, X isoform
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
  • DEAD/H box-3
  • Helicase-like protein 2
  • helicase like protein 2
Background InformationDDX3X, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, is a member of the DEAD box family of proteins. Characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), DEAD box proteins are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3X is an ATP-dependent RNA helicase and acts as a co-factor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. DDX3X is also involved in HIV-1 replication and interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
References
Product Information
FormatSerum
Control
  • A431 cell lysate
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-DDX3X Antibody (Rabbit Polyclonal Antibody) validated in WB to detect DDX3X also known as ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic linear peptide corresponding to DDX3X.
HostRabbit
SpecificityThis antibody detects RNA-binding protein DDX3X.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq].
Gene Symbol
  • CAP-Rf
  • DBX
  • DDX14
  • DDX3
  • EC 3.6.1.-
  • HLP2
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

SUBUNIT STRUCTURE: Found in a complex with Rev and XPO1. Interacts with XPO1 and TDRD3. Interacts with HCV core protein.

SUBCELLULAR LOCATION: Nucleus speckle. Cytoplasm. Note: Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.

SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.
Molecular Weight~73 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on A431 cell lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected DDX3X in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
09-860 04053252744440

Documentation

Anti-DDX3X Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-DDX3X Antibody Certificates of Analysis

TitleLot Number
Anti-DDX3X - 2430960 2430960
Anti-DDX3X - 2452490 2452490
Anti-DDX3X - 2325057 2325057
Anti-DDX3X - 3200651 3200651
Anti-DDX3X - 3321057 3321057
Anti-DDX3X - 3862683 3862683
Anti-DDX3X - NG1619915 NG1619915
Anti-DDX3X - NG1895575 NG1895575
Anti-DDX3X -2492823 2492823
Anti-DDX3X -2714517 2714517
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References

Reference overviewPub Med ID
Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells.
Vella, P; Barozzi, I; Cuomo, A; Bonaldi, T; Pasini, D
Nucleic acids research  40  3403-18  2012

Show Abstract 		22210892 22210892