Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB3404-C Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)

This Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytokeratins Gene Symbol: KRT18, CYK18, PIG46 UniProt Number: P05783
MAB3404-C
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, WB, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3404-C
Replaces04-586
DescriptionAnti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)
Alternate Names
  • Keratin, type I cytoskeletal 18
  • Cell proliferation-inducing gene 46 protein
  • Cytokeratin-18
  • CK-18
  • Keratin-18
  • K18
Background InformationCytokeratin 18, also known as Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, CK-18, Keratin-18, or K18, and encoded by the gene KRT18/CYK18/PIG46, is involved in the uptake of thrombin-anti-thrombin complexes by hepatic cells. When the protein is phosphorylated it plays a role in filament reorganization. Cytokeratin-18 together keratin-8 and IL-6 are involved in skin barrier protection too, and cytokeratin-18 is often complexed with Cytokeratin 8. Cytokeratin-18 is expressed in colon, placenta, liver epithelia and many other tissues. Cytokeratin 18 in epithelial cells is cleaved by caspases during apoptosis to produce apoptotic specific CK-18 fragment that is the basis of many apoptosis detection assays.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.
Key Applications
  • Immunohistochemistry
  • Western Blotting
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cytokeratin 18 in human placenta tissue.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Biological Information
ImmunogenCytoskeletal preparation of HeLa cells expressing human Cytokeratin 18.
CloneCK2
Concentration1 mg/mL
HostMouse
SpecificityThe antibody reacts with human cytokeratin 18 and is used for staining research samples of a wide variety of simple epithelia and simple glandular epithelia (e. g. intestinal, respiratory and urinary epithelia) and transitional epithelia (e. g. bladder), but stratified squamous epithelia are not stained (e. g. esophagus, epidermis).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KRT18
  • CYK18
  • PIG46
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~48 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cytokeratin 18 in human colorectal cancer tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3404-C 04053252975561

Documentation

Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) SDS

Title

Safety Data Sheet (SDS) 

Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) Certificates of Analysis

TitleLot Number
Anti-Cytokeratin 18, clone CK2 (Ascites Free) - Q2426055 Q2426055
Anti-Cytokeratin 18, clone CK2 (Ascites Free) - 3257010 3257010
Anti-Cytokeratin 18, clone CK2 (Ascites Free) - 3533572 3533572
Anti-Cytokeratin 18, clone CK2 (Ascites Free) - 3837033 3837033
Anti-Cytokeratin 18, clone CK2 (Ascites Free) -2720119 2720119
Anti-Cytokeratin 18, clone CK2 (Ascites Free) Monoclonal Antibody 2970992