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MABE873 Anti-Chd2, clone 8H3 Antibody

This Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.

Anti-Chd2, clone 8H3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: Chd2 UniProt Number: E9PZM4
MABE873
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IP, ICC, ChIPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE873
DescriptionAnti-Chd2, clone 8H3 Antibody
Alternate Names
  • SNF2 chromatin remodelling enzyme
  • Chromodomain Helicase DNA Binding Protein 2
  • Protein Chd2
Background InformationChd2, also known as Chromodomain-helicase-DNA-binding protein 2 (CHD-2), or ATP-dependent helicase CHD2, and encoded by the gene CHD2, belongs to the CHD family of proteins. This family is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes are chromatin remodeling proteins and alter gene expression by modifying the chromatin structure of a particular gene. The alteration affects the access of the transcriptional apparatus to it, and thus the gene’s expression. In the mouse Chd2 is expressed in heart, forebrain, extremities, and facial and dorsal regions during specific times of embryonic development. Chd2 null mice are embryonic lethal, and in humans, various mutations in CHD-2 gene appear to cause a variety of disorders and developmental defects. Other mutations associated with the CHD2 gene locus appear to cause childhood-onset epileptic encephalopathy (EEOC).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chd2 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Biological Information
ImmunogenRecombinant protein corresponding to mouse Chd2.
Clone8H3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Chd2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~211 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. Two known isoforms may be observed at 50 kDa and 200 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Chd2 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE873 04053252955228

Documentation

Anti-Chd2, clone 8H3 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Chd2, clone 8H3 Antibody Certificates of Analysis

TitleLot Number
Anti-Chd2, clone 8H3 - Q2383914 Q2383914
Anti-Chd2, clone 8H3 - 3218874 3218874
Anti-Chd2, clone 8H3 - 3407335 3407335
Anti-Chd2, clone 8H3 - 3738081 3738081
Anti-Chd2, clone 8H3 - 3810505 3810505
Anti-Chd2, clone 8H3 - 3978231 3978231
Anti-Chd2, clone 8H3 - 4077670 4077670
Anti-Chd2, clone 8H3 - 4125701 4125701
Anti-Chd2, clone 8H3 - 4169254 4169254
Anti-Chd2, clone 8H3 -2659636 2659636
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Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics