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MABT1504-100UG Anti-Calponin Antibody, clone CALP

Anti-Calponin, clone CALP , Cat. No. MABT1504, is a mouse monoclonal antibody that detects Calponin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Calponin Antibody, clone CALP MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CNN1 UniProt Number: P51911
MABT1504-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, PorcineWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1504-100UG
DescriptionAnti-Calponin Antibody, clone CALP
Alternate Names
  • Basic calponin
  • Calponin H1 smooth muscle
Background InformationCalponin-1 (UniProt: P51911; also known as Basic calponin, Calponin H1 smooth muscle) is encoded by the CNN1 gene (Gene ID:1264) in human. Calponin is an actin filament-associated regulatory protein that is expressed in smooth muscle and non-muscle cells of vertebrates. Three isoforms of Calponin have been reported that inhibit actin-activated myosin ATPase activity and stabilize the actin cytoskeleton. Calponin 1 is specifically expressed in smooth muscle cells is involved in fine-tuning of smooth muscle contractility. Calponin-2 is found in multiple cell types, including developing smooth muscles and Calponin-3 is found in the neurons, astrocytes and glial cells. Calponin-1 can bind to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 (and other calponins) has a calponin homology (CH) domain at its N-terminal (aa 28-131) that can function either as a part of actin-binding site or serve as a regulatory structure for actin-binding activity. It also has three calponin-like domains (aa 164-189; 204-229; and 243-268) next to the C-terminal region that are conserved in all calponins. The C-terminal segment varies among different calponins and has differentiated effects on weakening the binding of calponin to F-actin. Deletion of the C-terminal tail segment is shown to enhance the actin-binding and bundling activities of calponins. (Ref.: Liu, R., and Jin, J-P (2016). Gene 585(1); 143-153).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Calponin, clone CALP , Cat. No. MABT1504, is a mouse monoclonal antibody that detects Calponin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Calponin in human uterus and human prostate tissue sections.
Biological Information
ImmunogenCrude human uterine muscle extract.
CloneCALP or N3/16
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone CALP specifically detects human Calponin-1.
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Human
  • Porcine
Species Reactivity NoteHuman, Bovine, Porcine.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CNN1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~36 kDa observed; 33.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human aortic smooth muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Calponin in human aortic smooth muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1504-100UG 04054839507243